The effects of implant surface nanoscale features on osteoblast-specific gene expression
The effects of implant surface nanoscale features on osteoblast-specific gene expression
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DOI：
10.1016/j.biomaterials.2009.04.010
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发表时间：
2009-09-01
期刊：
影响因子：
14
通讯作者：
Cooper, Lyndon F.
中科院分区：
文献类型：
--
作者：
Mendonca, Gustavo;Mendonca, Daniela B. S.;Cooper, Lyndon F.
本研究探讨了纳米级种植体表面特征对成骨细胞分化的影响。采用溶胶-凝胶法制备了具有不同纳米尺度的钛片（20.0 × 1.0 mm），并用扫描电子显微镜、原子力显微镜和X射线光电子能谱仪进行了表征和分析。将人间充质干细胞（hMSC）在盘上培养3-28天。评估ALP、BSP、Runx 2、OCN、OPG和OSX mRNA的水平以及一组76个与骨生成相关的基因。形貌和化学评价证实纳米级特征仅存在于涂层表面上。与机加工（M）和酸蚀刻（Ac）相比，骨特异性mRNA在具有叠加纳米级特征的表面上增加。在第14天，锐钛矿（An）、金红石（Ru）、氧化铝（Al）和氧化锆（Zr）的OSX mRNA水平分别增加2倍、3.5倍、4倍和3倍。M和Ac的OSX表达水平接近基线水平。在第14天和第28天，对于具有纳米级涂覆特征的所有表面，BSP相对mRNA表达显著上调（对于AI增加高达45倍）。与M相比，PCR阵列显示AI涂层植入物的上调。OSX和BSP表达的增加代表了粘附在纳米结构涂层植入物表面的细胞的反应改善。此外，使用氧化铝产生的纳米结构表面显著增强了代表成骨细胞分化的hMSC基因表达。钛种植体基质上的纳米级特征可以通过改变粘附细胞反应来改善骨整合反应。(C)2009爱思唯尔有限公司保留所有权利。
This study investigated the influence of nanoscale implant surface features on osteoblast differentiation. Titanium disks (20.0 x 1.0 mm) with different nanoscale materials were prepared using sol-gel-derived coatings and characterized by scanning electron microscopy, atomic force microscopy and analyzed by X-ray Photoelectron Spectrometer. Human Mesenchymal Stem Cells (hMSCs) were cultured on the disks for 3-28 days. The levels of ALP, BSP, Runx2, OCN, OPG, and OSX mRNA and a panel of 76 genes related to osteogenesis were evaluated. Topographical and chemical evaluation confirmed nanoscale features present on the coated surfaces only. Bone-specific mRNAs were increased on surfaces with superimposed nanoscale features compared to Machined (M) and Acid etched (Ac). At day 14, OSX mRNA levels were increased by 2-, 3.5-, 4- and 3-fold for Anatase (An), Rutile (Ru), Alumina (AI), and Zirconia (Zr), respectively. OSX expression levels for M and Ac approximated baseline levels. At days 14 and 28 the BSP relative mRNA expression was significantly up-regulated for all surfaces with nanoscale coated features (up to 45-fold increase for AI). The PCR array showed an up-regulation on AI coated implants when compared to M. An improved response of cells adhered to nanostructured-coated implant surfaces was represented by increased OSX and BSP expressions. Furthermore, nanostructured surfaces produced using aluminum oxide significantly enhanced the hMSC gene expression representative of osteoblast differentiation. Nanoscale features on Ti implant substrates may improve the osseointegration response by altering adherent cell response. (C) 2009 Elsevier Ltd. All rights reserved.