Enzymatic detergent treatment protocol that reduces protease-resistant prion protein load and infectivity from surgical-steel monofilaments contaminated with a human-derived prion strain.
Enzymatic detergent treatment protocol that reduces protease-resistant prion protein load and infectivity from surgical-steel monofilaments contaminated with a human-derived prion strain.
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酶洗涤剂处理方案可减少蛋白酶抗性朊病毒蛋白负载和被人源朊病毒菌株污染的手术钢单丝的感染性。
DOI：
10.1099/vir.0.82961-0
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
C. Masters
中科院分区：
文献类型：
--
作者：
V. Lawson;James D. Stewart;C. Masters
朊病毒疾病的传染因子的非常规性质对常规感染控制方法提出了挑战。变异型和散发性Creutzfeldt-Jakob病的神经组织分布增加了对通过普通外科手术传播朊病毒疾病风险的关注，并强调了可纳入常规处理的去污程序的必要性。在这项研究中，酶的医疗器械清洁剂的制剂，以减少与人类朊病毒病的啮齿动物适应株，以前报告的耐去污相关的感染性的能力进行了测试。通过酶清洁制剂有效降解疾病相关朊病毒蛋白需要高处理温度（50-60摄氏度）。标准去污方法（1 M NaOH 1 h或134 ℃高压灭菌18 min）可将人源性朊病毒菌株相关的感染性降低小于3 log 10 LD 50。相比之下，使用优化的酶清洁制备方案进行30 min处理可将感染性降低3 log 10 LD 50以上，并且当与高压灭菌器循环结合使用时，可消除可检测水平的感染性。开发与医疗和手术器械的常规清洁和灭菌兼容的朊病毒去污程序可能会降低普外科手术中朊病毒疾病传播的风险。
The unconventional nature of the infectious agent of prion diseases poses a challenge to conventional infection control methodologies. The extraneural tissue distribution of variant and sporadic Creutzfeldt-Jakob disease has increased concern regarding the risk of prion disease transmission via general surgical procedures and highlighted the need for decontamination procedures that can be incorporated into routine processing. In this study, the ability of preparations of enzymatic medical instrument cleaners to reduce the infectivity associated with a rodent-adapted strain of human prion disease, previously reported to be resistant to decontamination, was tested. Efficient degradation of the disease-associated prion protein by enzymatic cleaning preparations required high treatment temperatures (50-60 degrees C). Standard decontamination methods (1 M NaOH for 1 h or autoclaving at 134 degrees C for 18 min) reduced infectivity associated with the human-derived prion strain by less than 3 log10 LD50. In contrast, a 30 min treatment with the optimized enzymatic cleaning preparation protocols reduced infectivity by more than 3 log10 LD50 and when used in conjunction with autoclave cycles eliminated detectable levels of infectivity. The development of prion decontamination procedures that are compatible with routine cleaning and sterilization of medical and surgical instruments may reduce the risk of the transmission of prion disease in general surgery.