Diagnostic Value of Serum MiR-587 in Patients with Metabolic Syndrome
Diagnostic Value of Serum MiR-587 in Patients with Metabolic Syndrome
复制标题
血清miR-587对代谢综合征患者的诊断价值
DOI：
10.7754/clin.lab.2019.181253
复制
发表时间：
2019-01-01
影响因子：
0.7
通讯作者：
Li, Jian
中科院分区：
文献类型：
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作者：
Guo, Jun;Lin, Yajun;Li, Jian
背景资料：为探讨代谢综合征（MS）患者血清miR-587水平的变化及其临床诊断价值，采用RT-qPCR方法检测50例高血糖患者、50例高脂血症患者和50例健康对照者血清miR-587水平。采用ROC曲线分析和MS相关性分析检测血清miR-587的诊断价值，采用双荧光素酶报告基因分析确定miR-587可能的靶基因。RT-qPCR结果显示，高血糖、高血脂患者血清miR-587相对含量分别为0.45 ± 0.30、0.41 ± 0.30，而健康人为1 ± 0.87。高血糖患者血清miR-587的ROC曲线下面积（AUC）为0.830（95%CI = 0.716 - 0.863，p < 0.001），敏感性为68.6%，特异性为89.3%。高脂血症患者血清miR-587的AUC为0.790（95%CI = 0.759 ~ 0.851，p < 0.001），敏感性为78%，特异性为89.7%。相关分析显示，血清miR-587水平与空腹血糖（FBG）呈负相关（r =-0.291，p < 0.05），与总胆固醇（TC）呈负相关（r =-0.243，p < 0.01）。结论：miR-587在MS患者血清中的表达降低是MS患者潜在的诊断指标和独立的危险因素。
Background: The current study aims to explore the changes of serum miR-587 level in patients with metabolic syndrome (MS) and analyze its clinical diagnostic value.Methods: The serum levels of miR-587 in 50 patients with hyperglycemia, 50 patients with hyperlipidemia, and 50 healthy controls were detected by RT-qPCR. The diagnostic value of serum miR-587 was detected by ROC analysis and correlation analysis in MS. Dual luciferase reporter assay was carried out to determine the possible target gene of miR-587.Results: The results of RT-qPCR showed that the relative content of serum miR-587 in patients with hyperglycemia and hyperlipidemia was 0.45 +/- 0.30, 0.41 +/- 0.30, compared with 1 +/- 0.87 in healthy people. The area under ROC curve (AUC) of serum miR-587 in hyperglycemic patients was 0.830 (95% CI = 0.716 -0.863, p < 0.001), with the sensitivity of 68.6% and specificity of 89.3%. The AUC of serum miR-587 in hyperlipidemia patients was 0.790 (95% CI = 0.759 -0.851, p < 0.001), with the sensitivity of 78% and specificity of 89.7%. Correlation analysis showed that serum miR-587 level was negatively correlated with fasting blood glucose (FBG) (r = -0.291, p < 0.05), and negatively correlated with total cholesterol (TC) (r = -0.243, p < 0.01). Furthermore, dual luciferase reporter assay showed that PTEN was the target gene of miR-587.Conclusions: In summary the decreased expression of miR-587 in serum is a potential diagnostic marker and independent risk factor in patients with MS.