KINETIC AND ANTIGENIC CHARACTERIZATION OF ALTERED PROTEIN-SYNTHESIS BY CAMPYLOBACTER-JEJUNI DURING CULTIVATION WITH HUMAN EPITHELIAL-CELLS
KINETIC AND ANTIGENIC CHARACTERIZATION OF ALTERED PROTEIN-SYNTHESIS BY CAMPYLOBACTER-JEJUNI DURING CULTIVATION WITH HUMAN EPITHELIAL-CELLS
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DOI：
10.1093/infdis/168.4.948
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发表时间：
1993-10-01
影响因子：
6.4
通讯作者：
CIEPLAK, W
中科院分区：
文献类型：
--
作者：
KONKEL, ME;MEAD, DJ;CIEPLAK, W
空肠弯曲杆菌与INT 407细胞单层培养物的培养导致与在不存在上皮细胞的情况下培养的细菌相比新的或增强的许多蛋白质的合成。这些蛋白质被检测到后60分钟内加入细菌的上皮细胞培养物，其合成时间与空肠弯曲菌内化的增加。针对与INT 407细胞一起培养的细菌产生的兔抗血清识别9种蛋白质，这些蛋白质不被单独在培养基中培养的针对空肠弯曲杆菌的抗血清识别。前者的抗血清抑制空肠弯曲菌的内化，但不结合，在剂量依赖性的方式。结果表明，空肠弯曲菌在与上皮细胞共培养时合成的一种或多种蛋白质在促进内化中起作用。
Cultivation of Campylobacter jejuni with INT 407 cell monolayer cultures results in new or enhanced synthesis of a number of proteins compared with bacteria cultured in the absence of the epithelial cells. These proteins were detected within 60 min after the addition of the bacteria to the epithelial cell cultures, and their synthesis was temporally associated with an increase in C jejuni internalization. A rabbit antiserum raised against bacteria that were cultivated with INT 407 cells recognized nine proteins that were not recognized by an antiserum against C jejuni cultivated in medium alone. The former antiserum inhibited the internalization, but not the binding, of Campylobacter jejuni in a dose-dependent fashion. The results suggest that one or more of the proteins synthesized by C jejuni in response to cocultivation with epithelial cells plays a role in facilitating internalization.