Lung edema clearance: 20 Years of progress selected contribution: Mechanisms that may stimulate the resolution of alveolar edema in the transplanted human lung
Lung edema clearance: 20 Years of progress selected contribution: Mechanisms that may stimulate the resolution of alveolar edema in the transplanted human lung
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DOI：
10.1152/japplphysiol.00252.2002
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发表时间：
2002-11-01
影响因子：
3.3
通讯作者：
Matthay, MA
中科院分区：
文献类型：
--
作者：
Ware, LB;Fang, XH;Matthay, MA
方法鉴别排斥肺。在1998年9月1日至2000年9月30日期间，加州移植供体网络确定了因不符合标准选择标准而未用于肺移植的供体（14），且其近亲已知情同意进行研究。该研究方案得到了加州移植供体网络医学委员会的批准。肺切除术方案。在不使用肺保存液的情况下，采用标准技术将肺整体切除。肺在充气到供者的标准潮气量后，在摄氏4度被运送到我们的实验室。从肺切除到实验室重新加热的平均时间为6-8小时。为了测量血红蛋白以计算水干重比，在肺收获时从供者处获得血液样本。运输后，从右下肺取组织样本进行水干比的重量测量。其他研究使用了额外的叶（43）。总共研究了31名供者的肺。由于常规只有一个或两个肺叶可用于测量肺泡液清除率，因此每个供者只研究一种情况，要么是特布他林刺激肺泡液清除率（8名供者），要么是未经刺激的肺泡液清除率（23名供者）。非刺激清除率之前有过报道（43）。
METHODSIdentification of rejected lungs. Between September 1, 1998 and September 30, 2000, the California Transplant Donor Network identified donors whose lungs were not used for transplantation because of failure to meet the standard selection criteria (14) and whose next-of-kin had given informed consent for research. The study protocol was approved by the California Transplant Donor Network medical committee.Protocol for lung resection. The lungs were resected en bloc by standard techniques without the use of a pulmonary preservation solution. The lungs were transported to our laboratory at 4 C after inflation to the donor’s standard tidal volume. The average time from lung resection to rewarming in the laboratory was 6–8 h. For measurement of hemoglobin for the calculation of the water-to-dry weight ratio, a blood sample was obtained from the donor at the time of lung harvest. After transport, tissue samples were obtained from the right lower lung for gravimetric measure of the water-todry weight ratio. Additional lobes were used for other studies (43). Overall, lungs were studied from 31 donors. Because only one or two lobes were routinely available for measurements of alveolar fluid clearance, only one condition was studied per donor, either terbutaline-stimulated alveolar fluid clearance (8 donors) or, for comparison, unstimulated alveolar fluid clearance (23 donors). The rates of unstimulated clearance have been previously reported (43).