Caenorhabditis elegans inositol 5-phosphatase homolog negatively regulates inositol 1,4,5-triphosphate signaling in ovulation
Caenorhabditis elegans inositol 5-phosphatase homolog negatively regulates inositol 1,4,5-triphosphate signaling in ovulation
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DOI：
10.1091/mbc.02-01-0008
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发表时间：
2002-05-01
影响因子：
3.3
通讯作者：
Sternberg, PW
中科院分区：
文献类型：
--
作者：
Bui, YK;Sternberg, PW
秀丽隐杆线虫的排卵需要由表皮生长因子（EGF）受体同源物LET-23激活的肌醇1，4，5-三磷酸（IP 3）信号传导。我们产生了一个缺失突变的I型5-磷酸酶，IPP-5，并发现了一种新的排卵表型，即受精囊超伸吞噬两个卵母细胞每个排卵周期。IPP-5的时间和空间表达与其提出的成年受精囊中IP 3信号传导的抑制一致。ipp-5作用于let-23的下游，并与let-23介导的IP 3信号通路基因相互作用。我们推断，IPP-5负调节IP 3信号，以确保适当的受精囊收缩。
Ovulation in Caenorizabditis elegans requires inositol 1,4,5-triphosphate (IP3) signaling activated by the epidermal growth factor (EGF)-receptor homolog LET-23. We generated a deletion mutant of a type I 5-phosphatase, ipp-5, and found a novel ovulation phenotype whereby the spermatheca hyperextends to engulf two oocytes per ovulation cycle. The temporal and spatial expression of IPP-5 is consistent with its proposed inhibition of IP3 signaling in the adult spermatheca. ipp-5 acts downstream of let-23, and interacts with let-23-mediated IP3 signaling pathway genes. We infer that IPP-5 negatively regulates IP3 signaling to ensure proper spermathecal contraction.