Thymine metabolism in Pseudomonas aeruginosa strain 1: the presence of a salvage pathway.
Thymine metabolism in Pseudomonas aeruginosa strain 1: the presence of a salvage pathway.
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铜绿假单胞菌菌株 1 中的胸腺嘧啶代谢：挽救途径的存在。
DOI：
10.1099/00221287-128-7-1391
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
J. Loutit
中科院分区：
文献类型：
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作者：
A. Potter;D. Musgrave;J. Loutit
与其他嘧啶类化合物相比，铜绿假单胞菌对外源胸腺嘧啶的吸收非常缓慢，大部分胸腺嘧啶被细胞分解。甲氨蝶呤或甲氨蝶呤抑制胸腺嘧啶核苷的生物合成时，胸腺嘧啶回收途径的功能虽然低效，但这一途径的运行更为有效。铜绿假单胞菌回收胸腺嘧啶的机制与大肠杆菌和假单胞菌不同，因为DNA中没有胸腺嘧啶核苷。与嗜酸假单胞菌一样，铜绿假单胞菌也缺乏胸腺嘧啶核苷磷酸化酶活性。分离胸腺嘧啶营养缺陷症的尝试没有成功。
Exogenous thymine was found to be taken up very slowly by Pseudomonas aeruginosa in comparison to other pyrimidines, and most of it was catabolized by the cell. The existence of a functional, although inefficient, thymine salvage pathway was demonstrated and this pathway operated more effectively when de novo thymidine nucleotide biosynthesis was inhibited by trimethoprim or methotrexate. The mechanism of thymine salvage by P. aeruginosa appears to be different from that of Escherichia coli and Pseudomonas acidovorans as thymidine was not incorporated into the DNA. Like P. acidovorans, P. aeruginosa lacked thymidine phosphorylase activity. Unsuccessful attempts were made to isolate thymine auxotrophs.