Deep sequencing analysis of RNAs from a grapevine showing Syrah decline symptoms reveals a multiple virus infection that includes a novel virus
Deep sequencing analysis of RNAs from a grapevine showing Syrah decline symptoms reveals a multiple virus infection that includes a novel virus
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DOI：
10.1016/j.virol.2009.02.028
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发表时间：
2009-05-10
期刊：
影响因子：
3.7
通讯作者：
Rowhani, A.
中科院分区：
文献类型：
--
作者：
Al Rwahnih, M.;Daubert, S.;Rowhani, A.
在寻找与西拉葡萄衰退症状相关的病毒时，我们使用生命科学454高通量测序对植物总RNA序列进行了分析。67.5从反转录的cDNA片段中获得了大量的序列数据，并筛选了病毒或类病毒来源的序列。数据显示，表现出衰退症状的葡萄树支持包括七种不同RNA基因组的混合感染。被鉴定为来自病毒或类病毒的片段跨越了类似于一万倍的相对流行范围，从48，278个来自Rupestris stern pitting相关病毒的片段到4个来自澳大利亚葡萄藤类病毒的片段。1527个片段被鉴定为来自所有未知的马拉非病毒。其完整的基因组进行测序和表征，并开发了RT-PCR测试，以分析其持有的分布，并证明其存在于叶蝉（marafiviruses的载体）从患病的葡萄树收集。最初的调查在加州的葡萄种植区发现了有限的病毒。(C)2009 Elsevier Inc. All rights reserved.
In a search for viruses associated with decline symptoms of Syrah grapevines, we have undertaken an analysis of total plant RNA sequences using Life Sciences 454 high-through put sequencing. 67.5 megabases of sequence data were derived from reverse-transcribed cDNA fragments, and screened for sequences of viral or viroid origin. The data revealed that a vine showing decline symptoms Supported a mixed infection that included seven different RNA genomes. Fragments identified as derived from viruses or viroids spanned a similar to ten thousand fold range in relative prevalence, from 48,278 fragments derived from Rupestris stern pitting-associated virus to 4 fragments from Australian grapevine viroid. 1527 fragments were identified as derived from all unknown marafivirus. Its complete genome was sequenced and characterized, and an RT-PCR test was developed to analyze its held distribution and to demonstrate its presence in leafhoppers (vector for marafiviruses) collected from diseased vines. Initial surveys detected a limited presence of the virus in grape-growing regions of California. (C) 2009 Elsevier Inc. All rights reserved.