Metabolic alterations of nucleotides and nucleic acids in HeLa cells infected with vaccinia virus.
Metabolic alterations of nucleotides and nucleic acids in HeLa cells infected with vaccinia virus.
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感染牛痘病毒的 HeLa 细胞中核苷酸和核酸的代谢变化。
DOI：
10.7883/yoken1952.12.405
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发表时间：
1959
期刊：
影响因子：
--
通讯作者：
I. Tagaya
中科院分区：
文献类型：
--
作者：
C. Nishimura;I. Tagaya
关于可溶性抗原的产生，Tagaya和Oda(1958)详细报道了痘苗病毒在HeLa细胞中的生长方式。用重接种量感染HeLa细胞，6h后细胞匀浆中首次出现可测补体结合抗原，5h后可检测到另一种可溶性抗原血凝素。几乎在同一时间，当血凝素在细胞匀浆中可测到时，细胞内感染病毒的滴度显著上升。基于利用电子显微镜对痘病毒细胞内发育的形态观察，Higashi等人。(1959)报告说，在感染3小时后，基质出现在细胞质中，在感染后6小时收获的细胞中，在基质区域内观察到了第一种可识别的病毒形式，即所谓的发育形式。噬菌体感染细菌生化分析的进展刺激了动物病毒的相同研究工作，但由于动物病毒和动物细胞所固有的困难，还没有出现那么多的报告，如脊髓灰质炎病毒(Maosa b等人，1957；Miroff等人，1957；Ackermann等人，1959；Salzman和Lockart，1959)、单纯疱疹病毒(Newton and Stoker，1958)和腺病毒(Ginsberg and Dixon，1959)。关于痘苗病毒，Magee和Sagik(1959)报道了C14-胸腺嘧啶核苷被掺入了痘苗病毒感染的HeLa细胞的DNA组分中，Chang(1959)在缺乏碳酸氢盐的组织培养实验中得出结论，核糖核酸可能在痘苗病毒的合成中发挥重要作用。在这篇通讯中，作者提供了关于感染痘苗病毒的HeLa细胞代谢变化的数据。实验结果表明，在感染早期，核糖核酸组分中放射性同位素磷的最大掺入时间推迟了数小时。此外，标记核苷酸的化学分析表明，与对照细胞的相应部分相比，感染HeLa细胞的胞苷-二磷酸和腺苷-三磷酸部分的放射性显著增加，而腺苷-一磷酸的情况则相反。
In special reference to the production of the soluble antigens, the details on the mode of growth of vaccinia virus in HeLa cells were reported by Tagaya and Oda (1958). When HeLa cells were infected with a heavy inoculum, the first appearance of measurable complement fixing antigen in the cell homogenate was 6 hours following infection and another soluble antigen, hemagglutinin, became measurable 5 hours later. A significant rise in titer of infective virus within cells was noticed at nearly the same time, when hemagglutinin became measurable in the cell homogenate. Based on the morphological observations of the intracellular development of poxviruses by use of electronmicroscope, Higashi et al. (1959) reported that matrix appeared in cytoplasm 3 hours following infection and that the first recognizable viral form, so-called developmental form, was observed within the matrix area in the cells harvested 6 hours after infection. Advance in the biochemical analyses of phage-infected bacteria stimulated the same line of research works in animal viruses, but due to the difficulties inherent in animal viruses and animal cells, not so many reports have ever appeared, i. e. on poliovirus (Maassab et al., 1957; Miroff et al., 1957; Ackermann et al., 1959; Salzman and Lockart, 1959), herpes simplex virus (Newton and Stoker, 1958) and adenovirus (Ginsberg and Dixon, 1959). As regards vaccinia virus, Magee and Sagik (1959) reported that C14-thymidine was incorporated into the DNA fraction of vaccinia-infected HeLa cells, and Chang (1959) concluded from the experiments on bicarbonate-deficient tissue culture that ribonucleic acid might play an important role on the synthesis of vaccinia virus. In this communication the authors present the data concerning the metabolic alterations in HeLa cells infected with vaccinia virus. The experimental results indicate that a marked incorporation of radioisotope phosphorus into acid-soluble fraction was observed in the early stage of infection and that the maximal incorporation into ribonucleic acid fraction was delayed by several hours. Furthermore, chemical analyses of labeled nucleotides demonstrate that the radioactivity was considerably increased in the cytidine-diphosphate and adenosine-triphosphate fractions of the infected HeLa cells as compared with that in the corresponding fractions of the control cells, while the situation was contrary with adenosine-monophosphate.