Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food
Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food
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DOI：
10.1128/aem.68.2.576-581.2002
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发表时间：
2002-02-01
影响因子：
4.4
通讯作者：
Meng, JH
中科院分区：
文献类型：
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作者：
Schroeder, CM;Zhao, CW;Meng, JH
对 1985 年至 2000 年间从人类、牛、猪和食品中回收的总共 361 株大肠杆菌 O157 菌株进行了检查，以更好地了解这些生物体中抗菌药物耐药性的流行情况。根据肉汤微量稀释结果，220 株 (61%) 分离株对所有 13 种测试的抗菌药物敏感。然而，99 株（27%）分离株对四环素耐药，93 株（26%）对磺胺甲恶唑耐药，61 株（17%）对头孢噻吩耐药，48 株（13%）对氨苄西林耐药。耐药频率最高的是猪菌株（n = 70），其中 52 株（74%）对磺胺甲恶唑耐药，50 株（71%）对四环素耐药，38 株（54%）对头孢噻吩耐药，17 株（24%）对氨苄西林耐药。根据 PCR 测定的志贺毒素基因的存在情况，210 个（58%）分离株被鉴定为产志贺毒素大肠杆菌 (STEC)。其中，耐药性普遍较低，其中对磺胺甲恶唑耐药21例（10%），对四环素耐药19例（9%）。根据乳胶凝集结果，189株（52%）菌株被鉴定为大肠杆菌O157:H7，其中19株（10%）对磺胺甲恶唑耐药，16株（8%）对四环素耐药。数据表明，使用四环素衍生物、磺胺类药物、头孢菌素和青霉素所施加的选择压力，无论是在人类和兽医医学中的治疗作用还是在动物生产环境中的预防作用，都是 STEC 和非 STEC O157 中抗菌素耐药性选择的关键驱动力。
A total of 361 Escherichia coli O157 isolates, recovered from humans, cattle, swine, and food during the years 1985 to 2000, were examined to better understand the prevalence of antimicrobial resistance among these organisms. Based on broth microdilution results, 220 (61%) of the isolates were susceptible to all 13 antimicrobials tested. Ninety-nine (27%) of the isolates, however, were resistant to tetracycline, 93 (26%) were resistant to sulfamethoxazole, 61 (17%) were resistant to cephalothin, and 48 (13%) were resistant to ampicillin. Highest frequencies of resistance occurred among swine isolates (n = 70), where 52 (74%) were resistant to sulfamethoxazole, 50 (71%) were resistant to tetracycline, 38 (54%) were resistant to cephalothin, and 17 (24%) were resistant to ampicillin. Based on the presence of Shiga toxin genes as determined by PCR, 210 (58%) of the isolates were identified as Shiga toxin-producing E. coli (STEC). Among these, resistance was generally low, yet 21 (10%) were resistant to sulfamethoxazole and 19 (9%) were resistant to tetracycline. Based on latex agglutination, 189 (52%) of the isolates were identified as E. coli O157:H7, among which 19 (10%) were resistant to sulfamethoxazole and 16 (8%) were resistant to tetracycline. The data suggest that selection pressure imposed by the use of tetracycline derivatives, sulfa drugs, cephalosporins, and penicillins, whether therapeutically in human and veterinary medicine or as prophylaxis in the animal production environment, is a key driving force in the selection of antimicrobial resistance in STEC and non-STEC O157.