Protective effects of Polygonum multiflorum on ischemic stroke rat model analysed by 1H NMR metabolic profiling
Protective effects of Polygonum multiflorum on ischemic stroke rat model analysed by 1H NMR metabolic profiling
复制标题
H-1 NMR代谢谱分析何首乌对缺血性中风大鼠模型的保护作用
DOI：
10.1016/j.jpba.2018.03.049
复制
发表时间：
2018-06-05
影响因子：
3.4
通讯作者：
Wang, Jun-Song
中科院分区：
文献类型：
--
作者：
Li, Ming-Hui;Ruan, Ling-Yu;Wang, Jun-Song
中风是大多数工业化国家的第三大常见死因。何首乌（Polygonum multiflorum，HSW）是一种具有多种药理活性的传统中药，在中药方剂中应用广泛。本研究旨在探讨HSW对缺血性脑卒中大鼠模型的保护作用及其机制。采用死亡率、神经功能缺损程度、脑梗死面积、组织病理学、免疫组化、生化指标、实时定量聚合酶链反应和蛋白质印迹法评价HSW对缺血性脑卒中的治疗作用。基于质子核磁共振（H-1 NMR）的代谢组学分析表明，HSW可通过改善脑缺血再灌注大鼠能量和氨基酸代谢紊乱，减轻活性氧引起的氧化应激，减轻炎症反应，从而减轻脑缺血再灌注损伤。HSW治疗增加了细胞抗氧化剂的水平，其通过核红细胞2相关因子2信号通路在缺血-再灌注期间清除活性氧，并通过降低炎症因子如环氧合酶-2、白细胞介素-1 β、白细胞介素-6和肿瘤坏死因子-α的水平发挥抗炎作用。整合代谢组学方法显示了其在理解HSW缓解缺血性卒中机制方面的潜力。HSW作为治疗缺血性脑卒中的有效药物有待进一步研究。(C)2018爱思唯尔B. V.保留所有权利。
Stroke is the third most common cause of death in most industrialized countries. Polygonum multiflorum (He-Shou-Wu, HSW) is one of the traditional Chinese medicines with multiple pharmacological activities which is widely used in Chinese recipe. This study aims to explore the protective effect of HSW on ischemic stroke rat model and to elucidate the underlying mechanisms. The mortality rate, neurological deficit, cerebral infarct size, histopathology, immunohistochemistry, biochemical parameters, quantitative real-time polymerase chain reaction and western blotting were used to access the treatment effects of HSW on ischemic stroke. Proton nuclear magnetic resonance (H-1 NMR) based metabolomics analysis disclosed that HSW could relieve stroke rats suffering from the ischemia/reperfusion injury by ameliorating the disturbed energy and amino acids metabolisms, alleviating the oxidative stress from reactive oxygen species and reducing the inflammation. HSW treatment increased levels of cellular antioxidants that scavenged reactive oxygen species during ischemia-reperfusion via the nuclear erythroid 2-related factor 2 signaling pathway, and exert anti-inflammatory effect by decreasing the levels of inflammatory factors such as cyclooxygenase-2, interleukin-1 beta, interleukin-6 and tumor necrosis factor-alpha. The integrated metabolomics approach showed its potential in understanding mechanisms of HSW in relieving ischemic stroke. Further study to develop HSW as an effective therapeutic agent to treat ischemic stroke is warranted. (C) 2018 Elsevier B.V. All rights reserved.