Bacterial reduction of selenium in coal mine tailings pond sediment.
Bacterial reduction of selenium in coal mine tailings pond sediment.
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DOI：
10.2134/jeq2006.0357
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发表时间：
2007-05
影响因子：
2.4
通讯作者：
T. Siddique;J. Arocena;R. Thring;Yiqiang Zhang
中科院分区：
文献类型：
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作者：
T. Siddique;J. Arocena;R. Thring;Yiqiang Zhang
评估了煤尾矿固体储存设施的沉积物通过本地细菌群落减少硒 (Se) 的能力。从沉积物中分离出 1 株 Se(6+) 还原菌 Enterobacter hormaechei (Tar11) 和 4 种 Se(4+) 还原菌肺炎克雷伯菌 (Tar1)、荧光假单胞菌 (Tar3)、嗜麦芽寡养单胞菌 (Tar6) 和羊肠杆菌 (Tar8)。孵育5 d后测定Se(6+)时，霍马氏肠杆菌从流出物中去除了96%添加的Se(6+) (0.92 mg L(-1))。对红色沉淀物的分析表明，Se(6+) 还原导致形成 Se(0) 球形颗粒（<1.0 微米），如在扫描电子显微镜 (SEM) 下观察到并通过 EDAX 证实。进行硒形态分析以检查添加或不添加霍马氏肠杆菌细胞的沉积物中添加的 Se(6+) 的命运。超过 99% 的添加 Se(6+)（约 2.5 mg L(-1)）在未灭菌沉积物（不含霍马氏肠杆菌细胞）以及灭菌（热灭活）沉积物（含有霍马氏肠杆菌细胞）中转化。本研究结果表明，矿区泻湖沉积物中含有 Se(6+)- 和 Se(4+)- 还原细菌，可能是可溶性硒（Se(6+) 和 Se(4+)）的重要汇。从金属污染沉积物中分离出的霍马肠杆菌可能在去除工业废水中的硒方面具有潜在的应用。
Sediment from a storage facility for coal tailings solids was assessed for its capacity to reduce selenium (Se) by native bacterial community. One Se(6+)-reducing bacterium Enterobacter hormaechei (Tar11) and four Se(4+)-reducing bacteria, Klebsiella pneumoniae (Tar1), Pseudomonas fluorescens (Tar3), Stenotrophomonas maltophilia (Tar6), and Enterobacter amnigenus (Tar8) were isolated from the sediment. Enterobacter hormaechei removed 96% of the added Se(6+) (0.92 mg L(-1)) from the effluents when Se(6+) was determined after 5 d of incubation. Analysis of the red precipitates showed that Se(6+) reduction resulted in the formation of spherical particles (<1.0 microm) of Se(0) as observed under scanning electron microscope (SEM) and confirmed by EDAX. Selenium speciation was performed to examine the fate of the added Se(6+) in the sediment with or without addition of Enterobacter hormaechei cells. More than 99% of the added Se(6+) (approximately 2.5 mg L(-1)) was transformed in the nonsterilized sediment (without Enterobacter hormaechei cells) as well as in the sterilized (heat-killed) sediment (with Enterobacter hormaechei cells). The results of this study suggest that the lagoon sediments at the mine site harbor Se(6+)- and Se(4+)-reducing bacteria and may be important sinks for soluble Se (Se(6+) and Se(4+)). Enterobacter hormaechei isolated from metal-contaminated sediment may have potential application in removing Se from industrial effluents.