A SALICYLATE-HYPOCHLORITE METHOD FOR DETERMINING AMMONIA IN SEAWATER
A SALICYLATE-HYPOCHLORITE METHOD FOR DETERMINING AMMONIA IN SEAWATER
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DOI：
10.1139/f80-106
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发表时间：
1980-01-01
影响因子：
2.4
通讯作者：
HOLMHANSEN, T
中科院分区：
文献类型：
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作者：
BOWER, CE;HOLMHANSEN, T
在硝普钠存在下，NH_3与水杨酸盐和次氯酸盐反应生成靛酚蓝，用比色法测定人工海水中的总NH_4-N。水杨酸盐-次氯酸盐法和苯酚-次氯酸盐法的10-1000 μ g NH 4-N/l的回收率比较好（r [相关系数] = 0.99）;对于水杨酸盐-次氯酸盐法，25 μ g NH 4-N/l及以上的变异系数小于3%。由于不使用苯酚，新方法比苯酚-次氯酸盐法更安全，但由于光敏性，它可能不适合现场测定。通过增加NH3浓度产生的特征颜色使该测定可用于直接、目视估计[动物]培养系统中的NH3。
Total NH4-N in artificial seawater was determined spectrophotometrically by a method in which indophenol blue is produced by the reaction of NH3 with salicylate and hypochlorite in the presence of sodium nitroprusside. Recoveries of 10-1000 .mu.g NH4-N/l by the salicylate- and phenol-hypochlorite methods compared well (r [correlation coefficient] = 0.99); the coefficient of variation for 25 .mu.g NH4-N/l and above was less than 3% for the salicylate-hypochlorite method. The new method is safer than the phenol-hypochlorite method because phenol is not used, but it may not be suitable for field determinations because of photosensitivity. The characteristic colors produced by increasing concentrations of NH3 make the assay useful for the direct, visual estimation of NH3 in [animal] culture systems.