CYTO-TOXICITY OF PLANT-LECTINS FOR MOUSE EMBRYONAL CARCINOMA-CELLS
CYTO-TOXICITY OF PLANT-LECTINS FOR MOUSE EMBRYONAL CARCINOMA-CELLS
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DOI：
10.1007/bf01534848
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
STANLEY, P
中科院分区：
文献类型：
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作者：
DRABER, P;STANLEY, P
检测了 10 种具有不同碳水化合物识别特性的植物凝集素对多种小鼠胚胎癌 (EC) 细胞系（F9、OTF9-63、PCC4、PCC3/A/1、P19 和 P19S1801A1）的细胞毒性。六种凝集素在 ≤ 浓度下对大多数细胞类型都具有毒性。 100μg/ml并且应该可用作分离EC糖基化突变体的直接选择剂。杀死 90% 细胞群所需的各种凝集素浓度在 EC 细胞系之间存在显着差异，最大的差异是来自普通小麦（小麦胚芽凝集素；WGA）和加纳单花 (GS-1) 的凝集素。不同 EC 系的凝集素结合能力也有所不同，并且不一定与其相对凝集素敏感性相关。某些甚至在200μg/ml浓度下也无毒的凝集素仍然在细胞表面表现出显着的结合。 EC 细胞系之间凝集素敏感性和凝集素结合能力的广泛差异可以诊断其各自细胞表面不同碳水化合物结构的表达。结果表明，所检查的 EC 系将产生不同的糖基化突变体家族。
The cytotoxicity of 10 plant lectins with different carbohydrate recognition properties towards a number of mouse embryonal carcinoma (EC) cell lines (F9, OTF9-63, PCC4, PCC3/A/1, P19 and P19S1801A1) was examined. Six of the lectins are toxic for the majority of the cell types at concentrations of .ltoreq. 100 .mu.g/ml and should be useful as direct selective agents for the isolation of EC glycosylation mutants. The concentration of the various lectins required to kill 90% of the cell population differs markedly between EC cell lines, the greatest variation being observed with the lectins from Triticum vulgaris (wheat germ agglutinin; WGA) and Griffonia simpliciflora (GS-1). The lectin-binding abilities of different EC lines also vary and do not necessarily correlate with their relative lectin sensitivities. Certain lectins which are not toxic even at concentrations of 200 .mu.g/ml nevertheless exhibit significant binding at the cell surface. The extensive variation in lectin sensitivities and lectin-binding abilities between the EC cell lines is diagnostic of the expression of different carbohydrate structures at their respective cell surfaces. The results suggest that the EC lines examined will generate different families of glycosylation mutants.