LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1 OVEREXPRESSION AND T-CELL AUTOREACTIVITY
LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1 OVEREXPRESSION AND T-CELL AUTOREACTIVITY
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DOI：
10.1002/art.1780370915
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发表时间：
1994-09-01
影响因子：
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通讯作者：
OROURKE, K
中科院分区：
文献类型：
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作者：
RICHARDSON, B;POWERS, D;OROURKE, K
目标。目的：探讨DNA甲基化抑制剂是否通过诱导淋巴细胞功能相关抗原1(LFA-1)(CD11a/CD18)过表达而使T细胞发生自身反应。用3种不同的DNA甲基化抑制剂处理T细胞克隆，或将CD18基因稳定地导入哺乳动物表达载体，检测其对LFA-1表达和激活要求的影响。LFA-1的过度表达，无论是由DNA甲基化抑制剂引起的，还是由转基因引起的，都与自身反应性的发生有关。LFA-1过表达可能与T细胞自身反应性有关。
Objective. To determine if DNA methylation inhibitors make T cells autoreactive by inducing lymphocyte function-associated antigen type 1 (LFA-1) (CD11a/ CD18) overexpression.Methods. T cell clones were treated with 3 distinct DNA methylation inhibitors or were stably transfected with a CD18 cDNA in a mammalian expression vector, and the effects on LFA-1 expression and activation requirements were examined.Results. LFA-1 overexpression, caused by DNA methylation inhibitors or by transfection, correlates with the development of autoreactivity.Conclusion. LFA-1 overexpression may contribute to T cell autoreactivity.