Combining nano-physical and computational investigations to understand the nature of "aging" in dermal collagen.
Combining nano-physical and computational investigations to understand the nature of "aging" in dermal collagen.
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DOI：
10.2147/ijn.s121400
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发表时间：
2017
影响因子：
8
通讯作者：
Bozec L
中科院分区：
文献类型：
--
作者：
Ahmed T;Nash A;Clark KE;Ghibaudo M;de Leeuw NH;Potter A;Stratton R;Birch HL;Enea Casse R;Bozec L
真皮的细胞外基质是一个复杂的、动态的系统，随着年龄的增长，真皮的各种成分经历着不同的生理变化。特别是通过原子力显微镜纳米压痕测量老化对胶原原纤维纳米力学性能的影响，特别是通过测量老化对胶原原纤维纳米机械性能的影响，研究了与年龄相关的胶原物理性质的变化。随着年龄的增长，横纹纤维的杨氏模数出现下降(青年志愿者和老年志愿者的杨氏模数分别从8.11 Gpa降至4.19 Gpa，P<；0.001)。据推测，这是由于与年龄相关的纤维内水分保持的差异导致纤维密度的变化。通过电子结构计算对新的胶原蛋白-水相互作用机理进行了验证，表明其在能量上是可行的。
The extracellular matrix of the dermis is a complex, dynamic system with the various dermal components undergoing individual physiologic changes as we age. Age-related changes in the physical properties of collagen were investigated in particular by measuring the effect of aging, most likely due to the accumulation of advanced glycation end product (AGE) cross-links, on the nanomechanical properties of the collagen fibril using atomic force microscope nano-indentation. An age-related decrease in the Young’s modulus of the transverse fibril was observed (from 8.11 to 4.19 GPa in young to old volunteers, respectively, P<0.001). It is proposed that this is due to a change in the fibril density caused by age-related differences in water retention within the fibrils. The new collagen–water interaction mechanism was verified by electronic structure calculations, showing it to be energetically feasible.