Poliovirus induces apoptosis in the human U937 promonocytic cell line
Poliovirus induces apoptosis in the human U937 promonocytic cell line
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DOI：
10.1006/viro.2000.0405
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发表时间：
2000-07-05
期刊：
影响因子：
3.7
通讯作者：
Carrasco, L
中科院分区：
文献类型：
--
作者：
López-Guerrero, JA;Alonso, M;Carrasco, L
对脊髓灰质炎病毒感染中等易感的人前单核细胞U937细胞系已被用来研究这种病毒诱导细胞凋亡的作用。脊髓灰质炎病毒感染U937细胞可引起典型的细胞凋亡的形态改变。已分离出对脊髓灰质炎病毒耐药的U937细胞(PRU)，这些细胞对脊髓灰质炎病毒诱导的细胞凋亡具有抵抗力，但经肿瘤坏死因子加放线菌素治疗后发生凋亡。尽管脊髓灰质炎病毒可在U937细胞中触发一氧化氮的产生，但诱导型一氧化氮合酶(NO)的抑制剂N-omega-单甲基-L-精氨酸并未阻止感染后的细胞凋亡，表明NO在这一过程中不起直接作用。最后，脊髓灰质炎病毒感染U937细胞后，caspase-3原和聚(ADP-核糖)聚合酶裂解，表明CPP32 ICE样半胱氨酸蛋白酶在诱导细胞凋亡中被激活。我们的发现表明，感染脊髓灰质炎病毒的U937细胞由于细胞凋亡而发生细胞死亡，并涉及caspase激活。(C)2000年学术出版社。
The human promonocytic U937 cell line, which is moderately susceptible to poliovirus infection, has been used to investigate the induction of apoptosis by this virus. infection of U937 cells with poliovirus induces morphological changes typical of apoptosis. Poliovirus-resistant U937 cells (PRU) have been isolated that are resistant to apoptosis induced by poliovirus, but that undergo apoptosis after treatment with TNF plus cycloheximide. Despite the fact that poliovirus triggers nitric oxide production in U937 cells, the inhibitor of inducible nitric oxide (NO) synthase, N-omega-monomethyl-L-arginine, did not hinder apoptosis after infection, suggesting that NO does not play a direct role in this process. Finally, poliovirus infection of U937 cells led to the cleavage of pro-caspase-3 and poly(ADP-ribose)polymerase, indicating the activation of the CPP32 ICE-like cysteine protease in the induction of apoptosis. Our findings suggest that cellular death takes place in U937 cells productively infected by poliovirus as a result of apoptosis and involves caspase activation. (C) 2000 Academic Press.