Circadian signaling in the Northern krill Meganyctiphanes norvegica: In silico prediction of the protein components of a putative clock system using a publicly accessible transcriptome
Circadian signaling in the Northern krill Meganyctiphanes norvegica: In silico prediction of the protein components of a putative clock system using a publicly accessible transcriptome
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DOI：
10.1016/j.margen.2017.09.001
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发表时间：
2018-02-01
期刊：
影响因子：
1.9
通讯作者：
Pascual, Micah G.
中科院分区：
文献类型：
--
作者：
Christie, Andrew E.;Yu, Andy;Pascual, Micah G.
北磷虾是北大西洋许多区域浮游动物群落的重要组成部分。在其栖息地，褐家鼠具有非常重要的生态意义，因为它既是浮游植物/小型浮游动物的主要消费者，也是较高级别消费者的主要食物来源。对捕食者和捕食者的躲避和取食都有重要意义的一种行为是垂直迁移(DVM)，即黄昏时从深处上升，黎明时返回深海。在这类和其他类中，内源性的昼夜节律起搏器被认为至少在一定程度上控制了DVM。目前，还没有关于组成褐家鼠昼夜节律系统的基因/蛋白质的身份的信息。事实上，关于甲壳类生物昼夜节律的分子基础的信息通常很少。在这里，一个可公开访问的转录组被用来鉴定一个假定的巨藻昼夜节律系统的分子组成。一套完整的核心时钟蛋白是从褐家牛转录组(时钟、隐色素2、周期、周期和永恒)推导出来的，以及一大套可能作为核心时钟的调节器(例如双倍时间)、或作为核心时钟的输入(隐色素1)或作为其输出(色素分散激素)的蛋白质。这是首次描述了一个“完整的”(通过假定的输出通路信号的核心时钟)真虾类时钟系统，并因此为启动褐家鼠和其他磷虾物种昼夜节律信号的分子研究提供了基础，包括时钟系统如何调节DVM和其他行为。
The Northern krill Meganyctiphanes norvegica is a significant component of the zooplankton community in many regions of the North Atlantic Ocean. In the areas it inhabits, M. norvegica is of great importance ecologically, as it is both a major consumer of phytoplankton/small zooplankton and is a primary food source for higher-level consumers. One behavior of significance for both feeding and predator avoidance in Meganyctiphanes is diel vertical migration (DVM), i.e., a rising from depth at dusk and a return to depth at dawn. In this and other euphausiids, an endogenous circadian pacemaker is thought, at least in part, to control DVM. Currently, there is no information concerning the identity of the genes/proteins that comprise the M. norvegica circadian system. In fact, there is little information concerning the molecular underpinnings of circadian rhythmicity in crustaceans generally. Here, a publicly accessible transcriptome was used to identify the molecular components of a putative Meganyctiphanes circadian system. A complete set of core clock proteins was deduced from the M. norvegica transcriptome (clock, cryptochrome 2, cycle, period and timeless), as was a large suite of proteins that likely function as modulators of the core clock (e.g., doubletime), or serves as inputs to it (cryptochrome 1) or outputs from it (pigment dispersing hormone). This is the first description of a "complete" (core clock through putative output pathway signals) euphausiid clock system, and as such, provides a foundation for initiating molecular investigations of circadian signaling in M. norvegica and other krill species, including how clock systems may regulate DVM and other behaviors.