Effect of Stromal Vascular Fractions on Angiogenesis of Injected Diced Cartilage.
Effect of Stromal Vascular Fractions on Angiogenesis of Injected Diced Cartilage.
复制标题
DOI：
10.1097/scs.0000000000007996
复制
发表时间：
2022-03-01
期刊：
影响因子：
--
通讯作者：
Liao JL
中科院分区：
文献类型：
--
作者：
Yin H;Xie C;Li L;Zeng L;Zhu ZW;Chen N;Xie HJ;Liao JL
本研究探讨了脂肪源性基质血管成分（SVFs）对注射自体软骨块中血管生成的影响。通过酶消化提取基质维管组分。从新西兰兔的一侧耳软骨收获软骨移植物，然后切成1.0 mm 3的大小。将移植物分为2组。对照组为软骨块与培养液混合，实验组为软骨块与SVFs混合。将两组复合移植物分别植入每只家兔背部两侧皮下。术后4、12、24周观察移植物组织结构、血管数量及血管生成因子。 SVF符合生物学评价的现行标准。倒置显微镜下观察，4周时实验组软骨细胞层数明显多于对照组。 软骨移植物周围可见少量炎性细胞和血管。在12周和24周，通过一般观察，组织体积逐渐增加。 光镜下可见大量软骨细胞，炎性细胞减少。同时观察到额外的新血管。CD 31免疫组化分析显示，对照组毛细血管数在4、12、24周时均明显低于实验组。 RT-PCR和Western blot分别检测缺氧诱导因子-1（HIF-1）和血管内皮生长因子（VEGF）mRNA和蛋白的表达。结果显示，实验组VEGF和HIF-1α mRNA表达均增加。24周时mRNA水平仍高于对照组（P <0.05）。 实验组VEGF和HIF-1α蛋白的相对表达水平在4、12、24周均高于对照组（P <0.05）。 自体软骨与脂肪来源的SVFs混合注射移植时可以促进血管生成。进一步的研究表明，SVFs可提高移植软骨中VEGF和HIF-1α的表达水平，这可能是SVFs促进切割软骨血管生成的部分机制。
This study explored the effect of adipose-derived stromal vascular fractions (SVFs) on angiogenesis in injected autologous diced cartilage. Stromal vascular fractions were extracted by enzymatic digestion. Cartilage grafts were harvested from 1 side of the auricular cartilage of New Zealand rabbit and then diced to a size of 1.0 mm3. The grafts were divided into 2 groups. The control group was diced cartilage mixed with culture medium, and the experimental group was diced cartilage mixed with SVFs. The 2 groups of composite grafts were subcutaneously implanted on both sides of the back of each rabbit. After 4, 12 and 24 weeks, the tissue structure, number of blood vessels, and angiogenic factors in the grafts were observed. The SVFs conformed to the current standard of the biological evaluation. Under an inverted microscope, the number of layers of chondrocytes in the experimental group was higher than that in the control group at 4 weeks. A small number of inflammatory cells and blood vessels were observed around the cartilage grafts. At 12 and 24 weeks, the volume of tissue was increased gradually by general observation. And a large number of chondrocytes were observed microscopically, whereas the number of inflammatory cells decreased. And meanwhile additional new blood vessels were observed. Immunohistochemical analysis of CD31 showed that the number of capillaries in the control group was significantly lower than that in the experimental group at 4, 12 and 24 weeks. Further, the expression of Hypoxia inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) mRNA and protein were measured by RT-PCR and Western bloting, respectively. The results showed that the mRNA expression of VEGF and HIF-1α in the experimental group was increased. The mRNA level remained higher than that of the control group at 24 weeks (P < 0.05). And the relative expression levels of VEGF and HIF-1α protein in the experimental group were higher than those in the control group at 4, 12 and 24 weeks (P < 0.05). Autologous diced cartilage mixed with adipose-derived SVFs can promote angiogenesis when transplanted by injection. Further research showed that SVFs could increase the expression levels of VEGF and HIF-1α in the grafts, which may be part of the mechanism that SVFs promoted the angiogenesis of diced cartilage.