Insights into the circadian rhythm alterations of the novel PFOS substitutes F-53B and OBS on adult zebrafish.
Insights into the circadian rhythm alterations of the novel PFOS substitutes F-53B and OBS on adult zebrafish.
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DOI：
10.1016/j.jhazmat.2023.130959
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发表时间：
2023-02
影响因子：
13.6
通讯作者：
Qiyu Wang;Xueyan Gu;Yu Liu;Shuai Liu;Wuting Lu;Yongming Wu;Huiqiang Lu;Jing Huang;W. Tu
中科院分区：
文献类型：
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作者：
Qiyu Wang;Xueyan Gu;Yu Liu;Shuai Liu;Wuting Lu;Yongming Wu;Huiqiang Lu;Jing Huang;W. Tu
作为全氟辛烷磺酸（PFOS）的替代品，6：2 Cl-PFESA（F-53 B）和全氟壬烯氧基苯磺酸钠（OBS）在水生环境中经常被检测到，但人们对它们的神经毒性知之甚少，特别是在昼夜节律方面。本研究以斑马鱼昼夜节律-多巴胺（DA）调控网络为切入点，对1 μM PFOS、F-53 B和OBS进行了为期21 d的慢性暴露，比较研究了它们的神经毒性及其机制。结果表明，全氟辛烷磺酸可能会影响对热的反应，而不是昼夜节律，减少DA的分泌，由于中断钙信号通路转导引起的中脑肿胀。相反，F-53 B和OBS改变了成年斑马鱼的昼夜节律，但它们的作用机制不同。具体而言，F-53 B可能通过干扰氨基酸神经递质代谢和破坏血脑屏障（BBB）的形成来改变昼夜节律，而OBS主要通过减少室管膜细胞纤毛的形成来抑制经典Wnt信号转导，并诱导中脑脑室扩大，最终触发DA分泌失衡和昼夜节律改变。我们的研究强调，需要重点关注全氟辛烷磺酸替代品的环境暴露风险及其多种毒性的顺序和相互作用机制。
As alternatives to perfluorooctane sulfonate (PFOS), 6:2 Cl-PFESA (F-53B) and sodiump-perfluorous nonenoxybenzene sulfonate (OBS) are frequently detected in aquatic environments, but little is known about their neurotoxicity, especially in terms of circadian rhythms. In this study, adult zebrafish were chronically exposed to 1 μM PFOS, F-53B and OBS for 21 days taking circadian rhythm-dopamine (DA) regulatory network as an entry point to comparatively investigate their neurotoxicity and underlying mechanisms. The results showed that PFOS may affect the response to heat rather than circadian rhythms by reducing DA secretion due to disruption of calcium signaling pathway transduction caused by midbrain swelling. In contrast, F-53B and OBS altered the circadian rhythms of adult zebrafish, but their mechanisms of action were different. Specifically, F-53B might alter circadian rhythms by interfering with amino acid neurotransmitter metabolism and disrupting blood-brain barrier (BBB) formation, whereas OBS mainly inhibited canonical Wnt signaling transduction by reducing cilia formation in ependymal cells and induced midbrain ventriculomegaly, finally triggering imbalance in DA secretion and circadian rhythm changes. Our study highlights the need to focus on the environmental exposure risks of PFOS alternatives and the sequential and interactive mechanisms of their multiple toxicities.