Parallel flow arteriovenous shunt for the ex vivo evaluation of heparinized materials.
Parallel flow arteriovenous shunt for the ex vivo evaluation of heparinized materials.
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用于肝素化材料的离体评估的平行流动静脉分流器。
DOI：
10.1002/jbm.820190206
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Sefton,MV
中科院分区：
文献类型：
--
作者：
Ip,WF;Zingg,W;Sefton,MV
在使用旨在避免手术伪影的新型平行流动静脉分流器的犬中，发现肝素-聚乙烯醇（hep-PVA）涂层聚乙烯管路的通畅性显著长于聚乙烯醇（PVA）涂层但不含肝素的对照管路。使用标准Silastic慢性分流管（3.18 mm i.d.）插入狗的髂动脉和静脉之间。2周恢复期后，将小直径涂层聚乙烯管（1.14 mm i.d.）通过一对硅橡胶Y型连接器与慢性分流管的外置部分平行连接，因此<3% of the shunt flow was diverted into the test tube. The chronic shunt was reused many times over a >通畅期为6个月，无需频繁手术，并减少了结果的动物间差异。在较高的主分流流量下，肝素化和对照管路之间的通畅性差异更大，表明Y型接头对血小板粘附或聚集有显著影响。这种效应表现为循环血小板计数的时间依赖性降低。闭塞后肝素化管中部的扫描电子显微镜（SEM）检查表明不存在血小板和纤维蛋白沉积，与不含肝素的对照管不同。尽管Y型连接器发挥了重要作用，但它们并没有主导该分流管中发生的血栓形成过程，因此可以证明固定化肝素的生物学有效性。
The patency of heparin‐polyvinyl alcohol (hep‐PVA) coated polyethylene tubing was found to be significantly longer than control tubes coated with polyvinyl alcohol (PVA) but without heparin at low flow rates in dogs using a novel parallel flow arteriovenous shunt designed to avoid surgical artifacts. A standard Silastic chronic shunt (3.18 mm i.d.) was inserted between the iliac artery and vein of a dog. After a 2‐week recovery period, a small diameter coated polyethylene tube (1.14 mm i.d.) was connected in parallel with the exteriorized portion of the chronic shunt through a pair of Silastic Y‐connectors, so that <3% of the shunt flow was diverted into the test tube. The chronic shunt was reused many times over a >6 month patency period, eliminating the need for frequent surgery and reducing interanimal variability in the results. The difference in patency between heparinized and control tubes was greater at higher mainshunt flow rates indicating the presence of a significant effect of the Y‐connectors on platelet adhesion or aggregation. This effect was manifested in a time‐dependent reduction in circulating platelet count. Scanning electron microscopy (SEM) examination of the midportion of the heparinized tubes after occlusion demonstrated the absence of platelet and fibrin deposits, unlike the control tubes without heparin. Although the Y‐connectors played a significant role, they did not dominate the thrombotic processes occurring in this shunt and consequently the biological effectiveness of the immobilized heparin could be demonstrated.