Simultaneous calcium recordings of hippocampal CA1 and primary motor cortex M1 and their relations to behavioral activities in freely moving epileptic mice
Simultaneous calcium recordings of hippocampal CA1 and primary motor cortex M1 and their relations to behavioral activities in freely moving epileptic mice
复制标题
海马 CA1 和初级运动皮层 M1 的同步钙记录及其与自由活动癫痫小鼠行为活动的关系
DOI：
10.1007/s00221-020-05815-w
复制
发表时间：
2020-05-18
影响因子：
2
通讯作者：
Shen, Hui
中科院分区：
文献类型：
--
作者：
Dong, Xi;Zhang, Xin;Shen, Hui
癫痫是一种以反复发作为特征的常见神经系统疾病。大多数癫痫病例的原因是未知的。以往虽然有关于癫痫发作时单个脑区钙事件变化的报道，但关于自由活动小鼠两个不同脑区钙事件与癫痫行为关系的研究较少。分析海马CA 1（CA 1）和初级运动皮层M1（M1）同时记录的钙事件，探讨其与自由活动癫痫模型各种癫痫行为的关系。用海人酸（KA）诱发C57/BL 6 J小鼠癫痫模型。用多通道纤维光度计同时记录钙指示剂标记的CA_1和M_1区神经元和胶质细胞的钙事件。KA诱发癫痫发作时出现3种典型的钙事件：钙基线升高、皮质扩散性抑制（CSD）和钙闪烁。结果表明，CA_1和M_1的钙基线升高是同步的，而CSD波则不同步。然而，在这两个地区的同步钙闪烁是不确定的，因为它只在CA 1检测到。我们还观察到不同的钙事件发生不同的癫痫行为。基线上升事件伴有前肢阵挛或震颤，CSD波与头部运动密切相关（15/18，6只小鼠）。钙闪烁明确发生剧烈惊厥性运动性癫痫发作（CMS，6只小鼠）。结果表明，海马CA 1区和M1区的钙事件存在同步性，不同的钙事件与不同的癫痫发作行为有关。我们的研究结果表明，钙事件参与癫痫的神经网络和行为的同步化。
Epilepsy is a common neurological disorder characterized by recurrent epileptic seizures. The cause of most cases of epilepsy is unknown. Although changes of calcium events in a single brain region during seizures have been reported before, there have been few studies on relations between calcium events of two different brain regions and epileptic behaviors in freely moving mice. To analyze calcium events simultaneously recorded in hippocampal CA1 (CA1) and primary motor cortex M1 (M1), and to explore their relations to various epileptic behaviors in freely moving epileptic models. Epileptic models were induced by Kainic acid (KA), a direct agonist of glutamatergic receptor, on adult male C57/BL6J mice. Calcium events of neurons and glia in CA1 and M1 labeled by a calcium indicator dye were recorded simultaneously with a multi-channel fiber photometry system. Three typical types of calcium events associated with KA-induced seizures were observed, including calcium baseline-rising, cortical spreading depression (CSD) and calcium flashing with a steady rate. Our results showed that the calcium baseline-rising occurred in CA1 was synchronized with that in M1, but the CSD waves were not. However, synchronization of calcium flashing in the two areas was uncertain, because it was only detected in CA1. We also observed that different calcium events happened with different epileptic behaviors. Baseline-rising events were accompanied by clonus of forelimbs or trembling, CSD waves were closely related to head movements (15 out of 18, 6 mice). Calcium flashing occurred definitely with drastic convulsive motor seizures (CMS, 6 mice). The results prove that the synchronization of calcium event exists in CA1 and M1, and different calcium events are related with different seizure behaviors. Our results suggest that calcium events involve in the synchronization of neural network and behaviors in epilepsy.