High-speed bubble-segmented blood sampler for the rat.
High-speed bubble-segmented blood sampler for the rat.
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大鼠高速分段气泡血液采样器。
DOI：
10.1152/jappl.1984.57.4.1284
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
G. Abbott
中科院分区：
文献类型：
--
作者：
M. Graham;G. Abbott
描述了一种用于清醒大鼠的动脉血采样器。使用该装置，可以以 1/s 的频率获取少量（10 微升）全血或血浆样本，并在注射化合物后的前 60-120 秒内得出准确的动脉时间浓度曲线，以进行局部血流或药代动力学测量。血液以恒定速率从植入的动脉导管抽入聚乙烯管中，同时通过侧通道定期将气泡引入血柱中。尽管在穿过管道时血液样本会发生一些分散，但这可以通过数学方法进行纠正。然而，如果感兴趣的信息是时间-浓度曲线下的面积，则无需进行校正。
An arterial blood sampler for use in the conscious rat is described. With this apparatus it is possible to obtain small (10 microliter) whole-blood or plasma samples as frequently as 1/s and to derive accurate arterial time-concentration curves in the first 60-120 s after compounds are injected for regional blood flow or pharmacokinetic measurements. The blood is withdrawn from an implanted arterial catheter into polyethylene tubing at a constant rate while bubbles are introduced at regular intervals via a side channel into the column of blood. Although some dispersion of blood samples occurs as the tubing is traversed, this can be mathematically corrected. However, correction is unnecessary if the information of interest is the area under the time-concentration curve.