Characterization of Serratia marcescens, S. liquefaciens, S. plymuthica and S. marinorubra by Electrophoresis of their Proteinases
Characterization of Serratia marcescens, S. liquefaciens, S. plymuthica and S. marinorubra by Electrophoresis of their Proteinases
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通过电泳鉴定粘质沙雷氏菌、液化沙雷氏菌、普城沙雷氏菌和海红沙雷氏菌的蛋白酶
DOI：
10.1099/00221287-99-2-301
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发表时间：
1977
期刊：
影响因子：
1.5
通讯作者：
F. Grimont
中科院分区：
文献类型：
--
作者：
P. Grimont;F. Grimont
摘要：62株粘质沙雷氏菌、24株S.产芽孢杆菌S. Alummuthica和S. marinorubra和其他属的27个代表性培养物一起进行琼脂凝胶电泳。蛋白酶活性通过酪蛋白沉淀反应证明。粘质沙雷氏菌产生1 ~ 4种阴极蛋白酶，其酶谱与菌株的生物群分类结果雅阁。产芽孢沙雷氏菌经常产生阳极和阴极蛋白酶，在一些生物群之间存在差异。沙门氏菌菌株产生一个共同的阴极蛋白酶，但没有阳极蛋白酶。S. marinorubra产生一种特殊的蛋白酶。研究中的其他细菌（胡萝卜软腐欧文氏菌、欧文氏菌和嗜水气单胞菌）产生的蛋白酶在电泳迁移率上不同于沙雷氏菌属的蛋白酶。结果表明，蛋白酶谱可用于沙雷氏菌属物种和生物群的鉴定。
SUMMARY: Supernatant fluids from 5-day cultures in skim milk nutrient broth of 62 strains of Serratia marcescens, 24 of S. liquefaciens, 20 of S. plymuthica and 22 of S. marinorubra, together with 27 representative cultures of other genera, were submitted to agar gel electrophoresis. Proteinase activity was demonstrated by the casein-precipitating reaction. Serratia marcescens produced one to four cathodic proteinases and the patterns were in good accord with the classification of strains into biovars. Serratia liquefaciens often produced anodic and cathodic proteinases with differences between some biovars. Serratia plymuthica strains produced one common cathodic proteinase but no anodic proteinase. Each of the three biovars of S. marinorubra produced a specific proteinase. Other bacteria in the study (Erwinia carotovora, Erwinia chrysanthemi and Aeromonas hydrophila) produced proteinases that differed from the Serratia enzymes in their electrophoretic mobility. The results indicate that proteinase patterns could be useful in the identification of Serratia species and biovars.