Molecular methodology in determining vaginal flora in health and disease: its time has come.
Molecular methodology in determining vaginal flora in health and disease: its time has come.
复制标题
DOI：
10.1007/s11908-005-0049-2
复制
发表时间：
2005-11-01
影响因子：
3.1
通讯作者：
Marrazzo, Jeanne M
中科院分区：
文献类型：
--
作者：
Fredricks, David N;Marrazzo, Jeanne M
人类阴道的微生物植物群可影响妇女、胎儿和新生儿的健康。传统的培养方法无法检测到一些挑剔的阴道细菌，导致普查不完整。分子生物学的最新进展促进了无需培养即可检测和鉴定细菌，并描述了这种方法的优点和局限性。对阴道植物群的分子研究发现了许多未培养的细菌物种。例如，梭菌目中的几种新细菌是细菌性阴道病的高度特异性指示剂，并且与巨球菌属、纤毛菌属、异菌属和小杆菌属物种相关的细菌通常在患有细菌性阴道病的受试者中发现。通过采用分子工具对阴道微生物种群进行更全面的了解，可能会导致更好的策略来维持健康的阴道菌群，并将为探索新细菌在生殖道疾病中的作用创造机会。
The microbial flora of the human vagina can affect the health of women, their fetuses, and newborns. Conventional cultivation methods fail to detect some fastidious vaginal bacteria, leading to an incomplete census. Recent advances in molecular biology have facilitated the detection and identification of bacteria without cultivation, and the advantages and limitations of this approach are described. Molecular studies of the vaginal flora have discovered many uncultivated bacterial species. For instance, several novel bacteria in the Clostridiales order are highly specific indicators of bacterial vaginosis, and bacteria related to Megasphaera, Leptotrichia, Atopobium, and Dialister species are commonly found in subjects with bacterial vaginosis. A more complete understanding of vaginal microbial populations resulting from the adoption of molecular tools may lead to better strategies to maintain healthy vaginal floras and will create opportunities to explore the role of novel bacteria in reproductive tract diseases.