EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA-1 IS INCREASED IN HUMAN VASCULAR RESTENOSIS LESIONS
EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA-1 IS INCREASED IN HUMAN VASCULAR RESTENOSIS LESIONS
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DOI：
10.1172/jci116027
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发表时间：
1992-10-01
影响因子：
15.9
通讯作者：
WEIR, L
中科院分区：
文献类型：
--
作者：
NIKOL, S;ISNER, JM;WEIR, L
在体内获得的人类动脉粥样硬化被用来测试的假设，即转化生长因子-β 1在血管restenosis.We的发展中发挥了作用，分析了28例标本，原发性动脉粥样硬化或再狭窄病变，其中26个是通过定向斑块旋切术和2在冠状动脉搭桥手术的时间。7个对照组织包括手术切除的人乳内动脉段、心肌和术中获得的静脉移植物未使用部分。从这35个标本，210切片进行了检查，使用原位杂交。银颗粒/细胞核的测量结果显示，再狭窄组织中转化生长因子-β 1 mRNA的表达最高（与原发性动脉粥样硬化组织相比，P < 0.001），而非动脉粥样硬化（对照）组织中的表达最低。在培养的人血管平滑肌细胞从外植体的内乳动脉，转化生长因子-β 1的mRNA的表达显着大于在汇合的平滑肌细胞亚汇合（P = 0.05）。转化生长因子β III型受体在细胞培养物中表达，在组织标本中检测不到。用抗转化生长因子-β 1和α-肌动蛋白的抗体，通过免疫组织化学对原位杂交研究的邻近切片进行检查（作为平滑肌细胞的标志物），并公开了存在于这些切片中的平滑肌细胞中的转化生长因子-β 1。这些发现与转化生长因子-β 1在调节血管损伤的修复中起重要作用的概念一致，包括球囊血管成形术后再狭窄。
Human atheromata obtained in vivo were used to test the hypothesis that transforming growth factor-beta1 plays a role in the development of vascular restenosis.We analyzed 28 specimens from patients with primary atherosclerotic or restenotic lesions; 26 of these were obtained by directional atherectomy and 2 at the time of coronary bypass surgery. Seven control tissues included operatively excised segments of human internal mammary artery, myocardium, and unused portions of vein graft obtained intraoperatively. From these 35 specimens, 210 sections were examined using in situ hybridization. Measurement of silver grains / nucleus disclosed that expression of transforming growth factor-beta1 mRNA was highest in restenotic tissues (P < 0.001 vs. primary atherosclerotic tissues) and lowest in nonatherosclerotic (control) tissues. In cultures of human vascular smooth muscle cells grown from explants of internal mammary artery, expression of mRNA for transforming growth factor-beta1 was significantly greater in subconfluent than in confluent smooth muscle cells (P = 0.05). Transforming growth factor type-beta III receptor was expressed in cell cultures and undetectable in the tissue specimens. Sections taken adjacent to those studied by in situ hybridization were examined by immunohistochemistry using antibodies against transforming growth factor-beta1 and alpha-actin (as a marker for smooth muscle cells) and disclosed transforming growth factor-beta1 in smooth muscle cells present in these sections.These findings are consistent with the concept that transforming growth factor-beta1 plays an important role in modulating repair of vascular injury, including restenosis, after balloon angioplasty.