Compartmentalization of the Precheliceral Neuroectoderm in the Spider Cupiennius salei: Development of the Arcuate Body, Optic Ganglia, and Mushroom Body
Compartmentalization of the Precheliceral Neuroectoderm in the Spider Cupiennius salei: Development of the Arcuate Body, Optic Ganglia, and Mushroom Body
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DOI：
10.1002/cne.22355
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发表时间：
2010-07-01
影响因子：
2.5
通讯作者：
Stollewerk, Angelika
中科院分区：
文献类型：
--
作者：
Doeffinger, Carola;Hartenstein, Volker;Stollewerk, Angelika
与脊椎动物类似，节肢动物的大脑被划分为具有特定神经功能的中枢，如认知、行为和记忆。这些中心可以进一步细分为更小的功能单元。这就提出了一个问题：这些隔室是如何在发育过程中形成的，以及它们是如何整合到大脑中心的。我们在这里首次展示了Cupiennius salei蜘蛛的螯前神经外胚层是如何被划分成视觉系统中不同的大脑中心的：视神经节、蘑菇体和弓形体。视觉脑中枢的区域由沟槽和囊泡的形成确定，表达前神经基因CsASH1，随后表达神经分化标记物普洛斯彼罗。此外，在果蝇的蘑菇体和外视神经节中富集的转录因子dachshund在蜘蛛的视神经原和蘑菇体中表达。发育中的大脑中心被进一步细分为单个神经前体群，它们被合并到凹槽和囊泡中，但在整个发育过程中仍然可区分，这表明它们编码了神经亚型识别的空间信息。蜘蛛和昆虫的视神经节和蘑菇体发育的几个分子和形态学方面是相似的。此外，我们发现初级头斑为中间眼的视神经节提供神经元，而在昆虫和甲壳类动物中与视神经节相关的次级头斑则缺失。[j] .中国生物医学工程学报，2016,18(5):532 - 532。(C) 2010 Wiley-Liss, Inc。
Similarly to vertebrates, arthropod brains are compartmentalized into centers with specific neurological functions such as cognition, behavior, and memory. The centers can be further subdivided into smaller functional units. This raises the question of how these compartments are formed during development and how they are integrated into brain centers. We show here for the first time how the precheliceral neuroectoderm of the spider Cupiennius salei is compartmentalized to form the distinct brain centers of the visual system: the optic ganglia, the mushroom bodies, and the arcuate body. The areas of the visual brain centers are defined by the formation of grooves and vesicles and express the proneural gene CsASH1, followed by expression of the neural differentiation marker Prospero. Furthermore, the transcription factor dachshund, which is strongly enriched in the mushroom bodies and the outer optic ganglion of Drosophila, is expressed in the optic anlagen and the mushroom bodies of the spider. The developing brain centers are further subdivided into single neural precursor groups, which become incorporated into the grooves and vesicles but remain distinguishable throughout development, suggesting that they encode spatial information for neural subtype identity. Several molecular and morphological aspects of the development of the optic ganglia and the mushroom bodies are similar in the spider and in insects. Furthermore, we show that the primary engrailed head spot contributes neurons to the optic ganglia of the median eyes, whereas the secondary head spot, which has been associated with the optic ganglia in insects and crustaceans, is absent. J. Comp. Neurol. 518:2612-2632, 2010. (C) 2010 Wiley-Liss, Inc.