A novel urease-negative Helicobacter species associated with colitis and typhlitis in IL-10-deficient mice.
A novel urease-negative Helicobacter species associated with colitis and typhlitis in IL-10-deficient mice.
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DOI：
10.1128/.67.4.1757-1762.1999
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发表时间：
1999
影响因子：
3.1
通讯作者：
James G. Fox;P. Gorelick;M. Kullberg;Z. Ge;F. Dewhirst;Jerrold M. Ward
中科院分区：
文献类型：
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作者：
James G. Fox;P. Gorelick;M. Kullberg;Z. Ge;F. Dewhirst;Jerrold M. Ward
从患有炎症性肠病的IL-10（白细胞介素-10）缺陷（IL-10(-/-)）小鼠的肠道中分离出一种具有双极单鞘鞭毛的螺旋形细菌。该菌为微需氧菌，生长于37℃和42℃，氧化酶和过氧化氢酶呈阳性，脲酶呈阴性。根据16S rRNA基因序列分析及生化和表型标准，将该生物归类为一种新型幽门螺杆菌。没有幽门螺杆菌感染的剖宫产后IL-10（-/-）小鼠没有肠道炎症的组织学证据。然而，不含螺杆菌的IL-10（-/-）、SCID/NCr和A/JNCr小鼠在实验中接种了新型脲酶阴性幽门螺杆菌后，下肠出现了不同程度的炎症反应，在免疫功能正常的小鼠中，实验感染伴随着对新型幽门螺杆菌抗原的免疫球蛋白G抗体的相应升高。这些数据支持了最近的其他研究，这些研究表明，在自然和实验感染的小鼠中，多种幽门螺杆菌都可以诱导炎症性肠病。幽门螺杆菌相关肠道炎症的小鼠模型对于理解特定的微生物抗原如何影响复杂的疾病过程是有价值的。
A spiral-shaped bacterium with bipolar, single-sheathed flagella was isolated from the intestines of IL-10 (interleukin-10)-deficient (IL-10(-/-)) mice with inflammatory bowel disease. The organism was microaerobic, grew at 37 and 42 degrees C, and was oxidase and catalase positive but urease negative. On the basis of 16S rRNA gene sequence analysis and biochemical and phenotypic criteria, the organism is classified as a novel helicobacter. Cesarean section-rederived IL-10(-/-) mice without helicobacter infection did not have histological evidence of intestinal inflammation. However, helicobacter-free IL-10(-/-), SCID/NCr, and A/JNCr mice experimentally inoculated with the novel urease-negative Helicobacter sp. developed variable degrees of inflammation in the lower intestine, and in immunocompetent mice, the experimental infection was accompanied by a corresponding elevated immunoglobulin G antibody response to the novel Helicobacter sp. antigen. These data support other recent studies which demonstrate that multiple Helicobacter spp. in both naturally and experimentally infected mice can induce inflammatory bowel disease. The mouse model of helicobacter-associated intestinal inflammation should prove valuable in understanding how specific microbial antigens influence a complex disease process.