Overexpression of SBPase enhances photosynthesis against high temperature stress in transgenic rice plants
Overexpression of SBPase enhances photosynthesis against high temperature stress in transgenic rice plants
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DOI：
10.1007/s00299-006-0299-y
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发表时间：
2007-09-01
影响因子：
6.2
通讯作者：
Zhu, Yingguo
中科院分区：
文献类型：
--
作者：
Feng, Lingling;Wang, Kun;Zhu, Yingguo
通过过量表达水稻植株9，311（Oryza sativa L.）水稻品种中花11的cDNA序列分析该基因工程使植物能够在叶绿体中积累SBR4，并导致幼苗生长期间对高温胁迫的耐受性增强。此外，转基因植株的CO2同化能力显著高于野生型植株。叶绿素荧光和SBR2含量及活性分析表明，高温对光合作用的增强与光系统II的功能无关，而与SBR2含量及活性有关。Western blotting分析表明，高温胁迫导致SBB1与基质组分的类囊体膜的关联。然而，这种关联在野生型植物中比在转基因植物中明显得多。本研究结果表明，高温胁迫下，SBB1通过阻止Rubisco活化酶从可溶性基质中分离到类囊体膜上，维持了Rubisco的活性，从而增强了CO2同化对高温胁迫的耐受性。结果表明，过量表达SBB1基因可能是提高植物耐高温性的一种有效方法。
Activity of the Calvin cycle enzyme sedoheptulose-1,7-bisphosphatase (SBPase) was increased by overexpression of a rice plants 9,311 (Oryza sativa L.) cDNA in rice plants zhonghua11 (Oryza sativa L.). The genetic engineering enabled the plants to accumulate SBPase in chloroplasts and resulted in enhanced tolerance to high temperature stress during growth of young seedlings. Moreover, CO2 assimilation of transgenic plants was significantly more tolerant to high temperature than that of wild-type plants. The analyses of chlorophyll fluorescence and the content and activation of SBPase indicated that the enhancement of photosynthesis to high temperature was not related to the function of photosystem II but to the content and activation of SBPase. Western blotting analyses showed that high temperature stress led to the association of SBPase with the thylakoid membranes from the stroma fractions. However, such an association was much more pronounced in wild-type plants than that in transgenic plants. The results in this study suggested that under high temperature stress, SBPase maintained the activation of ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) by preventing the sequestration of Rubisco activase to the thylakoid membranes from the soluble stroma fraction and thus enhanced the tolerance of CO2 assimilation to high temperature stress. The results suggested that overexpression of SBPase might be an effective method for enhancing high temperature tolerance of plants.