Gut microbiota of liver transplantation recipients.
Gut microbiota of liver transplantation recipients.
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肝移植受者的肠道微生物群
DOI：
10.1038/s41598-017-03476-4
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发表时间：
2017-06-19
影响因子：
4.6
通讯作者：
Zeng ZG
中科院分区：
文献类型：
--
作者：
Sun LY;Yang YS;Qu W;Zhu ZJ;Wei L;Ye ZS;Zhang JR;Sun XY;Zeng ZG
肠道微生物群落的特征可能会受到终末期肝病患者病理生理变化的影响。在这里，我们重点研究了肝移植（LT）后患者肠道粪便微生物群落的特征，并与相同个体的LT前和健康个体进行了比较。通过16 S核糖体RNA的V4区的MiSeq-PE 250测序分析粪便微生物群落，然后在组间进行比较。我们发现，等待LT的严重肝病患者的肠道微生物群与健康对照组显著不同，如第一主成分所示（p = 0.0066）。此外，第二主成分代表LT手术前和LT手术后患者肠道微生物群的显著差异（p = 0.03125）。LT后，某些微生物种类的丰度显著下降，如放线杆菌、埃希氏菌和志贺氏菌，而其他微生物种类的丰度显著增加，如小单孢菌科、脱硫菌目、真杆菌科的Sarcinagenus和阿克曼氏菌。基于KEGG谱，与LT前样本相比，LT后样本中富集了15个功能模块，而21个功能模块的代表性较低。我们的研究表明，粪便微生物群落显着改变LT。
The characteristics of intestinal microbial communities may be affected by changes in the pathophysiology of patients with end-stage liver disease. Here, we focused on the characteristics of intestinal fecal microbial communities in post-liver transplantation (LT) patients in comparison with those in the same individuals pre-LT and in healthy individuals. The fecal microbial communities were analyzed via MiSeq-PE250 sequencing of the V4 region of 16S ribosomal RNA and were then compared between groups. We found that the gut microbiota of patients with severe liver disease who were awaiting LT was significantly different from that of healthy controls, as represented by the first principal component (p = 0.0066). Additionally, the second principal component represented a significant difference in the gut microbiota of patients between pre-LT and post-LT surgery (p = 0.03125). After LT, there was a significant decrease in the abundance of certain microbial species, such asActinobacillus,Escherichia, andShigella, and a significant increase in the abundance of other microbial species, such as Micromonosporaceae, Desulfobacterales, theSarcinagenus of Eubacteriaceae, andAkkermansia. Based on KEGG profiles, 15 functional modules were enriched and 21 functional modules were less represented in the post-LT samples compared with the pre-LT samples. Our study demonstrates that fecal microbial communities were significantly altered by LT.