Efficient encapsulation with plug-triggered drop formation
Efficient encapsulation with plug-triggered drop formation
复制标题
DOI：
10.1103/physreve.84.031502
复制
发表时间：
2011-09-22
影响因子：
2.4
通讯作者：
Weitz, David A.
中科院分区：
文献类型：
--
作者：
Abate, Adam R.;Rotem, Assaf;Weitz, David A.
用微流控装置形成的单分散微滴可用于包裹细胞、微凝胶颗粒，甚至是额外的液滴。因此，这些技术对于从高通量生物学到单分散颗粒和胶囊合成的应用非常有用，这些应用需要对这些对象进行封装。然而，有效地将对象封装在所有Drop中是一项挑战；通常，对象的封装效率低下，导致许多填充不正确、不可用的Drop。这里，我们描述了一种允许非常高效的封装的现象。我们使用对象的流入来堵住Drop Maker喷嘴；外部相的持续注入会挤压出一滴，从而封装该对象；这恰好产生了每一滴中封装的一个对象。
Monodisperse microscale drops formed with microfluidic devices are useful for encapsulating cells, microgel particles, or even additional drops. These techniques are thus useful for applications ranging from high-throughput biology to monodisperse particle and capsule synthesis, which require encapsulation of such objects. However, it is challenging to efficiently encapsulate the objects in all drops; often, the objects are encapsulated inefficiently, resulting in many improperly filled, unusable drops. Here, we describe a phenomenon that allows very efficient encapsulation. We use the inflow of the object to plug the drop maker nozzle; the continued injection of the outer phase pinches off a drop, thereby encapsulating the object; this yields precisely one object encapsulated per drop.