THE STAPHYLOCOCCUS-AUREUS MUTATION PCRA3 LEADS TO THE ACCUMULATION OF PT181 REPLICATION INITIATION-COMPLEXES
THE STAPHYLOCOCCUS-AUREUS MUTATION PCRA3 LEADS TO THE ACCUMULATION OF PT181 REPLICATION INITIATION-COMPLEXES
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DOI：
10.1016/0022-2836(91)90927-x
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发表时间：
1991-10-20
影响因子：
5.6
通讯作者：
BASHEER, R
中科院分区：
文献类型：
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作者：
IORDANESCU, S;BASHEER, R
在携带pcrA3染色体突变的金黄色葡萄球菌细胞中，质粒pT181及其衍生物的拷贝数维持在减少的水平。在琼脂糖凝胶电泳过程中，它们的DNA有很大一部分作为切口DNA迁移。在该质粒DNA种类的表征中获得的结果表明，它代表复制起始复合物。这种复合物不能在野生型宿主中检测到。pcrA3细胞中存在的复制起始复合物在延迟后可恢复复制。从这些结果可以得出结论，pcrA3宿主突变影响了质粒pT181复制中紧接在复制起始复合物形成之后的一个步骤，并且在pcrA3中，该步骤成为质粒pT181复制的速率限制。
InStaphylococcus aureuscells carrying thepcrA3chromosomal mutation, plasmid pT181 and its derivatives were maintained at a reduced copy number. A significant proportion of their DNA migrated during agarose gel electrophoresis as nicked DNA. The results obtained in the characterization of this plasmid DNA species show that it represents replication initiation complexes. Such complexes could not be detected in a wild-type host. The replication initiation complexes present inpcrA3cells could resume replication after a lag. It was concluded from these results that thepcrA3host mutation affected a step in plasmid pT181 replication immediately following the formation of the replication initiation complex, and that inpcrA3this step became rate-limiting for plasmid pT181 replication.