Temporal sensing platform based on anodic dissolution of Ag and cathodic biocatalysis of oxygen reduction for Staphylococcus aureus detection
Temporal sensing platform based on anodic dissolution of Ag and cathodic biocatalysis of oxygen reduction for Staphylococcus aureus detection
复制标题
基于银阳极溶解和氧还原阴极生物催化的金黄色葡萄球菌检测的时间传感平台
DOI：
10.1016/j.foodchem.2022.132404
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发表时间：
2022
期刊：
影响因子：
8.8
通讯作者：
Songqin Liu
中科院分区：
文献类型：
--
作者：
Bin Chen;Qin Tao;Fanglin Qiao;Yunjie Fei;Yuanjian Liu;Xiaohui Xiong;Songqin Liu
建立了一种Ag@C混合双极电极（BPE）传感平台，用于金黄色葡萄球菌（S.金黄色葡萄球菌）。结合了Ag的阳极溶解和氧（O2）还原的阴极生物催化的优点，该策略显示出超低的检测限，低至10 CFU mL−1。由于Ag@C的形成完全淬灭了鲁米诺的电化学发光（ECL）发射，因此ECL发射恢复反映了阳极溶解的程度。同时，S.金催化阴极O2的电化学还原，降低阴极O2还原的过电位，从而增加阳极电子损失速率，促进银的溶解并恢复鲁米诺的电致发光发射。在恒电位条件下，通过监测S. aureuson阴极，S.由于电导率的微小差异，可以量化金色。
An Ag@C hybrid bipolar electrode (BPE) sensing platform has been established for the temporal detection ofStaphylococcus aureus(S. aureus) in food. Combining the advantages of anodic dissolution of Ag and cathodic biocatalysis of oxygen (O2) reduction, this strategy showed an ultralow detection limit down to 10 CFU mL−1. As the formation of Ag@C completely quenched the electrochemiluminescence (ECL) emission of luminol, the ECL emission recovery reflected the extent of anodic dissolution. Meanwhile,S. aureuscatalyzed the electrochemical reduction of O2at the cathode, reducing the overpotential for cathodic O2reduction and thus increasing the rate of anodic electron loss, facilitating Ag dissolution and restoring the ECL emission of luminol. When a constant potential was applied, through monitoring the ECL recovery time before and after the incubation ofS. aureuson the cathode,S. aureuscould be quantified due to the slight difference of the conductivity.