Immunoreactive fibroblast growth factor (FGF) in rat tissues: molecular weight forms and the effects of hypophysectomy.
Immunoreactive fibroblast growth factor (FGF) in rat tissues: molecular weight forms and the effects of hypophysectomy.
复制标题
大鼠组织中的免疫反应性成纤维细胞生长因子（FGF）：分子量形式和垂体切除术的影响。
DOI：
10.1016/0006-291x(85)91054-x
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发表时间：
1985
影响因子：
3.1
通讯作者：
Pigeon,P
中科院分区：
文献类型：
--
作者：
Mormède,P;Baird,A;Pigeon,P
在中性 pH 值下用稀 (0.15 M) 硫酸铵提取大鼠组织，并检查成纤维细胞生长因子免疫反应性 (ir-FGF) 的存在。在所有组织中都发现了大量的 Ir-FGF，但在肝脏和肾脏中的含量较高。凝胶过滤柱色谱显示存在 ir-FGF 的三个分子量峰，一个与空隙体积相关（肾脏提取物中的主要形式），另一个具有 150-180,000 Da 的表观分子量（血清中的主要形式），第三个为 70,000 Da（其他检查组织中的主要形式：垂体、脑、肝脏、脾和胸腺）。与从牛垂体纯化的真实 FGF 共洗脱没有免疫反应性，表明 FGF 存在于与结合分子和/或前体相关的中性提取物中。垂体切除术对组织中的ir-FGF没有重大影响，但血清中的ir-FGF含量有所增加。结果表明，FGF 在几种胚胎学、形态学和发育上不同的组织中广泛分布，并表明垂体不是 FGF 免疫反应性的主要来源，尽管它可以参与血浆水平的调节。
Rat tissues were extracted at neutral pH with dilute (0.15 M) ammonium sulfate and examined for presence of fibroblast growth factor immunoreactivity (ir-FGF). Ir-FGF was found in substantial amounts in all the tissues but higher amounts were found in liver and kidney. Gel filtration column chromatography showed the presence of three molecular weight peaks of ir-FGF, one associated with the void volume (the major form in kidney extract), another with an apparent molecular weight of 150–180,000 Da (the major form in serum) and a third one of 70,000 Da (the major form in the other tissues examined: pituitary, brain, liver, spleen and thymus). No immunoreactivity coeluted with authentic FGF purified from bovine pituitary glands, suggesting that FGF is present in neutral extracts in association with binding molecules and/or precursors. Hypophysectomy had no major effect on ir-FGF in the tissues, but increased its amount in serum. The results establish a wide distribution of FGF in several embryologically, morphologically and developmentally distinct tissues and indicate that the pituitary is not the major source of FGF immunoreactivity, although it can be involved in the regulation of plasma levels.