Decoupling Electronic versus Nuclear Photoresponse of Isolated Green Fluorescent Protein Chromophores Using Short Laser Pulses
Decoupling Electronic versus Nuclear Photoresponse of Isolated Green Fluorescent Protein Chromophores Using Short Laser Pulses
复制标题
DOI：
10.1103/physrevlett.117.243004
复制
发表时间：
2016-12-09
影响因子：
8.6
通讯作者：
Andersen, Lars H.
中科院分区：
文献类型：
--
作者：
Kiefer, Hjalte V.;Pedersen, Henrik B.;Andersen, Lars H.
利用飞秒激光脉冲在静电离子储存环中研究了绿色荧光蛋白的去质子化模型生色团的电子物理。激光脉冲的持续时间比内部转换的时间短得多，因此，避免了通常遇到的ns-激光脉冲的顺序多光子吸收的贡献。在单光子激发后，作用吸收最大值在S-0至S-1波段内从其原点在约490至450 nm处发生位移，这可以通过检测到的作用中涉及的不同的光物理来解释。
The photophysics of a deprotonated model chromophore for the green fluorescent protein is studied by femtosecond laser pulses in an electrostatic ion-storage ring. The laser-pulse duration is much shorter than the time for internal conversion, and, hence, contributions from sequential multiphoton absorption, typically encountered with ns-laser pulses, are avoided. Following single-photon excitation, the action-absorption maximum is shown to be shifted within the S-0 to S-1 band from its origin at about 490 to 450 nm, which is explained by the different photophysics involved in the detected action.