Structure of testicular angiotensin-converting enzyme. A segmental mosaic isozyme.
Structure of testicular angiotensin-converting enzyme. A segmental mosaic isozyme.
复制标题
DOI：
--
复制
发表时间：
1989-10
期刊：
影响因子：
--
通讯作者：
Ravi S. Kumar;J. Kusari;S. Roy;R. Soffer;Ganes C. Sen
中科院分区：
文献类型：
--
作者：
Ravi S. Kumar;J. Kusari;S. Roy;R. Soffer;Ganes C. Sen
从兔睾丸血管紧张素转换酶cDNA克隆的序列中推导出了该酶的全部氨基酸序列。在体外从该cDNA编码的mRNA翻译出预期分子量为84，000的蛋白质。所有先前确定的序列的7个胰蛋白酶肽从酶中存在的推导序列，从而确认的身份的蛋白质。从推导的序列看来，该蛋白质在氨基末端含有信号肽，在羧基末端附近含有疏水锚定结构域。用寡核苷酸探针（其序列代表cDNA的不同区域）进行的北方分析不仅揭示了编码睾丸和肺同工酶的mRNA之间具有广泛同源性的区域，而且还揭示了睾丸mRNA特有的5'端附近的一段序列。
The complete amino acid sequence of rabbit testicular angiotensin-converting enzyme has been deduced from the sequence of the corresponding cDNA clone. A protein of the expected molecular weight of 84,000 was translated in vitro from the mRNA encoded by this cDNA. All of the previously determined sequences of seven tryptic peptides from the enzyme are present in the deduced sequence, thus confirming the identity of the protein. From the deduced sequence it appears that the protein contains a signal peptide at the amino terminus and a hydrophobic anchoring domain near the carboxyl terminus. Northern analysis with oligonucleotide probes, whose sequences represented different regions of the cDNA, revealed not only the regions of extensive homology between the mRNAs encoding the testicular and the pulmonary isozymes but also a stretch of sequence near the 5' end unique to the testicular mRNA.