Cloning by insertional mutagenesis of a cDNA encoding Caenorhabditis elegans kinesin heavy chain.
Cloning by insertional mutagenesis of a cDNA encoding Caenorhabditis elegans kinesin heavy chain.
复制标题
通过插入诱变克隆编码秀丽隐杆线虫驱动蛋白重链的 cDNA。
DOI：
10.1073/pnas.90.19.9181
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发表时间：
1993
影响因子：
11.1
通讯作者：
J. Mancillas
中科院分区：
文献类型：
--
作者：
N. Patel;D. Thierry;J. Mancillas
在秀丽隐杆线虫中的另一个遗传位点，unc-116，在导致运动缺陷的突变的筛选中被鉴定。通过使用转座子插入突变体和基因组的物理和遗传图谱克隆UNC-116。cDNA序列预测了一个815个氨基酸的蛋白质。基于序列比较和二级结构预测，unc-116编码驱动蛋白重链的所有三个结构域：马达、柄和尾。虽然马达和尾部结构域与克隆的驱动蛋白重链的等同结构域具有高度的同一性，但茎的rodII结构域比先前报道的那些明显短，并且预计不会形成卷曲螺旋α-螺旋。对两株C.编码顺行运动分子UNC-116和UNC-104的线虫基因应该提供对驱动蛋白重链超家族的这些成员的体内功能的了解。
An additional genetic locus in Caenorhabditis elegans, unc-116, was identified in a screen for mutations resulting in defective locomotion. unc-116 was cloned by use of a transposon insertion mutant and the physical and genetic map of the genome. The cDNA sequence predicts an 815-amino acid protein. Based upon sequence comparison and secondary structure predictions, unc-116 encodes all three domains of the kinesin heavy chain: the motor, stalk, and tail. While the motor and tail domains have a high degree of identity to the equivalent domains of cloned kinesin heavy chains, the rodII domain of the stalk is significantly shorter than those previously reported and is not predicted to form a coiled-coil alpha-helix. Analysis of mutational defects in two C. elegans genes encoding anterograde motor molecules, unc-116 and unc-104, should provide insight into the in vivo functions of these members of the kinesin heavy chain superfamily.