Perforin expression and cytotoxic activity of sputum CD8+ lymphocytes in patients with COPD
Perforin expression and cytotoxic activity of sputum CD8+ lymphocytes in patients with COPD
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DOI：
10.1378/chest.125.1.71
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发表时间：
2004-01-01
期刊：
影响因子：
9.6
通讯作者：
Siafakas, NM
中科院分区：
文献类型：
--
作者：
Chrysofakis, G;Tzanakis, N;Siafakas, NM
背景资料：既往研究表明，吸烟者对吸烟的炎症反应与非吸烟者不同，而CD8（+）T淋巴细胞在这一反应中起着关键作用。目的：研究COPD患者气道腔CD8（+）淋巴细胞的细胞毒活性和穿孔素表达。36名患有COPD的男性吸烟者，25名不患有COPD的男性吸烟者和10名健康的非吸烟者参加了这项研究。用合适的单克隆抗体标记诱导痰样品的T淋巴细胞，并使用流式细胞术测量。结果：COPD吸烟者CD8（+）淋巴细胞的百分比和总数显著高于非COPD吸烟者（分别为p = 0.01和p = 0.005）或健康非吸烟者（分别为p = 0.02和p = 0.01）。与其他两组相比，COPD吸烟者中CD8（+）细胞中的穿孔素表达显著更高（p = 0.001）。COPD患者诱导痰中T细胞的细胞毒活性也明显高于其他两组。结论：COPD吸烟者痰中CD8（+）细胞不仅数量增加，而且高度活化，表达高水平的穿孔素。这些结果表明，CD8（+）T淋巴细胞在COPD的炎症过程中发挥了重要作用。
Background: Previous studies have shown that the inflammatory response to cigarette smoking differs between smokers who acquire COPD and those who do not, and the CD8(+) T-lymphocytes have been identified as a key player in this response.Objective: To investigate the cytotoxic activity and perforin expression of CD8(+) lymphocytes in the airway lumen of patients with COPD.Methods: Thirty-six male smokers with COPD, 25 male smokers without COPD, and 10 healthy nonsmokers participated in the study. T-lymphocytes of induced sputum samples were labeled with appropriate monoclonal antibodies and measured using flow cytometry. The cytotoxic activity of CD8(+) cells was defined by incubating them with specific target cells (K562).Results: The percentage and the total number of CD8(+) lymphocytes were significantly higher in COPD smokers compared to non-COPD smokers (p = 0.01 and p = 0.005, respectively) or to healthy nonsmokers (p = 0.02 and p = 0.01, respectively). Perforin expression in CD8(+) cells was significantly higher in smokers with COPD compared to the other two groups (p = 0.001). Increased cytotoxic activity of T cells was also observed in induced sputum of patients with COPD in comparison to the other two groups.Conclusion: CD8(+) cells are not only increased in number in sputum samples of smokers with COPD but are highly activated, expressing high levels of perforin. These findings suggest that CD8(+) T-lymphocytes play a significant role in the inflammatory process of COPD.