Biodegradation of beta-cypermethrin by two Serratia spp. with different cell surface hydrophobicity.
Biodegradation of beta-cypermethrin by two Serratia spp. with different cell surface hydrophobicity.
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DOI：
10.1016/j.biortech.2009.12.083
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发表时间：
2010-05
影响因子：
11.4
通讯作者：
Chen Zhang;Le Jia;Shenghui Wang;Jie Qu;Kang Li;Lili Xu;Yanhua Shi;Yanchun Yan
中科院分区：
文献类型：
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作者：
Chen Zhang;Le Jia;Shenghui Wang;Jie Qu;Kang Li;Lili Xu;Yanhua Shi;Yanchun Yan
沙雷氏菌属从活性污泥中分离出的菌株 JC1 和 JCN13 可以降解并利用高效氯氰菊酯 (beta-CP) 作为在微盐培养基 (MSM) 中生长的唯一碳源和能源。两种菌株均能有效降解β-CP，接种物总生物量为0.1-0.3g干重wtL−1，温度为20-38℃，pH 6-9，初始β-CP为25-1000mgL−1，并通过酯和二苯醚裂解进行代谢，产生3-苯氧基苯甲酸（3-PBA）和苯酚，然后将其完全矿化。使用响应面法 (RSM) 来优化降解条件。在各自的最佳降解条件下，菌株JC1可以在10天内降解92％的β-CP，菌株JCN13在4天内降解率达到89％。细胞表面疏水性（CSH）和生物降解实验表明，JCN13比JC1具有更高的疏水性和降解能力，这意味着菌株的高疏水性可以增强β-CP的降解。
Serratia spp. strain JC1 and JCN13, isolated from activated sludge, could degrade and utilize beta-cypermethrin (beta-CP) as the sole carbon and energy sources for growth in the minimal salt media (MSM). The two strains could effectively degrade beta-CP with total inocula biomass 0.1–0.3g dry wtL−1, at 20–38°C, pH 6–9, initial beta-CP 25–1000mgL−1and metabolize it by cleavage of ester and diphenyl ether to yield 3-phenoxybenzoic acid (3-PBA) and phenol, then completely mineralize it. Response surface methodology (RSM) was used to optimize degradation conditions. Under their own optimal degradation conditions, strain JC1 could degrade 92% beta-CP within 10days and the degradation rate of strain JCN13 reached 89% within 4days. Cell surface hydrophobicity (CSH) and biodegradation assays indicated that JCN13 has higher hydrophobicity and degradation ability than JC1, and it means the high hydrophobicity of strains could enhance the degradation of beta-CP.