Interleukin-1 alpha is released during transfection of keratinocytes.
Interleukin-1 alpha is released during transfection of keratinocytes.
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Interleukin-1 alpha 在角质形成细胞转染过程中释放。
DOI：
10.1111/1523-1747.ep12396885
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Blumenberg,M
中科院分区：
文献类型：
--
作者：
Komine,M;Freedberg,IM;Blumenberg,M
Keratinocytes are known to produce, store, and release IL-1α and therefore we suspected that the DNA-mediated cell transfection procedure may release the stored IL-1α from keratinocytes into the medium. Using enzyme-linked immunosorbent assay, we determined the IL-1α concentration in culture supernatants during keratinocyte transfection. The following transfection methods were compared: lipofection with lipofectACE and lipofectAMlNE (GIBCO), Ca3(PO4)2co-precipitation, and polybrene-dimethylsulfoxide (DMSO). The supernatants were collected immediately prior to transfection, after 5-h incubation with lipofectin or Ca3(PO4)2, and 24 and 48 h after transfection. In the polybrene-DMSO method, the supernatant was also collected immediately before and after DMSO shock. LipofectAMINE caused the highest release of IL-1α, whereas the lipofectACE and polybrene-DMSO mediated transfection with confluent cells released the least. The other two methods released intermediate levels of IL-1α. Our data indicate that a substantial amount of IL-1α is released during the keratinocyte transfection procedure, which can affect the results of transfection in studies of gene expression.
