Syringin alleviates ovalbumin-induced lung inflammation in BALB/c mice asthma model via NF-κB signaling pathway
Syringin alleviates ovalbumin-induced lung inflammation in BALB/c mice asthma model via NF-κB signaling pathway
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DOI：
10.1002/tox.23049
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发表时间：
2020-11-04
影响因子：
4.5
通讯作者：
Wang, Yan
中科院分区：
文献类型：
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作者：
Dai, Rui;Niu, Manman;Wang, Yan
哮喘是一种慢性变应性肺气道炎性疾病，其特征是白色血细胞浸润和炎性细胞因子的释放，其发病机制复杂。从各种药用植物中提取的紫丁香苷已被广泛用于治疗炎性疾病。因此，本研究旨在进一步探讨白藜芦醇苷对卵清蛋白（OVA）诱导的哮喘小鼠模型的保护作用。用三种剂量（25、50和100 mg/kg）的白藜芦醇苷腹腔内处理OVA致敏的BALB小鼠，通过免疫球蛋白E（IgE）水平、细胞因子水平、组织病理学评价炎症细胞计数、肺重量、亚硝酸盐（NO）水平、氧化应激生物标志物和基因标志物的改变来验证。白藜芦醇苷的治疗强烈降低了增加的IgE、炎性细胞因子、WBC计数，并恢复了抗氧化应激标志物OVA刺激的动物。此外，在组织病理学分析中证实了炎症和粘液产生的显著减少，这进一步通过抑制白藜芦醇苷对NF-κ B途径的激活来验证。这些结果表明，胡萝卜素可能通过调节NF-κ B B通路的活化来改善OVA诱导的小鼠的哮喘症状。
Asthma is an allergic chronic inflammatory disease of the pulmonary airways, characterized by the infiltration of white blood cells and release of inflammatory cytokines of complex pathways linked to its pathogenesis. Syringin extracted from various medicinal plants has been used extensively for the treatment of inflammatory diseases. Hence, this study was conducted to further explore the protective effects of the syringin in ovalbumin (OVA) induced-asthma mice model. OVA-sensitized BALB mice were treated intraperitonealy with three doses (25, 50 and 100 mg/kg) of the syringin which was validated by the alteration in the immunoglobulin E (IgE) levels, cytokines levels, histopathological evaluation inflammatory cell count, lung weight, nitrite (NO) levels, oxidative stress biomarkers and gene markers. The treatment of syringin intensely reduced the increased IgE, inflammatory cytokines, WBC count and restored the antioxidant stress markers OVA stimulated animals. In addition, a significant reduction in inflammation and mucus production was evidenced in histopathological analysis which was further validated by suppression NF-kappa B pathway activation by syringin. These results suggest that syringin may improve asthma symptoms in OVA-induced mice by modulating NF-kappa B pathway activation.