Stabilization of gold nanoparticles on glass surface with polydopamine thin film for reliable LSPR sensing
Stabilization of gold nanoparticles on glass surface with polydopamine thin film for reliable LSPR sensing
复制标题
用聚多巴胺薄膜稳定玻璃表面的金纳米颗粒，实现可靠的局域表面等离子体共振传感
DOI：
10.1016/j.jcis.2015.08.075
复制
发表时间：
2015-12-15
影响因子：
9.9
通讯作者：
Hu, Weihua
中科院分区：
文献类型：
--
作者：
Chen, Hongming;Zhao, Lei;Hu, Weihua
贵金属等离子体纳米结构上产生的局域表面等离子体共振（LSPR）已证明在无标记和原位生物传感方面具有引人注目的应用。局域表面等离子体共振生物传感的一种广泛使用的形式是将预先合成的等离子体胶体吸附在透明基底上，并在生物分子结合或解离时跟踪其表面等离子体带波长和/或强度变化。然而，这种形式受到支撑的等离子体胶体的光学不稳定性的影响，这主要源于支撑的胶体的分离和/或聚集。在这项工作中，我们报告了一种新的策略，以金纳米颗粒（AuNP）作为模型，在胺功能化玻璃上稳定支撑的等离子体胶体，以实现可靠的等离子体生物传感。在该策略中，探索了聚多巴胺（PDA）（一种受贻贝启发的聚合物薄膜），通过简单的浸没反应来涂覆金纳米颗粒附着的玻璃。 PDA薄膜不仅可以防止负载的AuNPs分离或聚集，而且还可以轻松固定生物探针以进行进一步的等离子体生物传感。同时，通过优化薄膜厚度，涂层 AuNP 的折射率 (RI) 灵敏度基本上得以保留，从而为 LSPR 传感提供了可靠的平台。 (C) 2015 Elsevier Inc. 保留所有权利。
Localized surface plasmon resonance (LSPR) arising on noble metal plasmonic nanostructures has demonstrated compelling applications for label-free and in situ biosensing. A widely-used format for LSPR biosensing is to adsorb pre-synthesized plasmonic colloids on transparent substrates and track their surface plasmon band wavelength and/or intensity changes upon biomolecular binding or dissociation. However, this format suffers from the optical instability of the supported plasmonic colloids, which mainly originates from the detachment and/or aggregation of the supported colloids. In this work we report a novel strategy to stabilize the supported plasmonic colloids, with gold nanoparticles (AuNPs) as a model, on amine-functionalized glass for reliable plasmonic biosensing. In this strategy, polydopamine (PDA), a mussel-inspired polymeric thin film was explored to coat the AuNPs-attached glass by using a simple immersion reaction. The PDA thin film not only prevents the supported AuNPs from detaching or aggregating, but also allows for facile immobilization of biological probes for further plasmonic biosensing. Meanwhile, the refractive index (RI)-sensitivity of the coated AuNPs was essentially preserved by optimizing the thickness of the thin film, thus offering a reliable platform for LSPR sensing. (C) 2015 Elsevier Inc. All rights reserved.