Characterization of two classes of ribonucleoprotein complexes possibly involved in RNA editing from Leishmania tarentolae mitochondria.
Characterization of two classes of ribonucleoprotein complexes possibly involved in RNA editing from Leishmania tarentolae mitochondria.
复制标题
可能参与利什曼原虫线粒体 RNA 编辑的两类核糖核蛋白复合物的表征。
作者：
M. Peris;G. Frech;A. M. Simpson;F. Bringaud;E. Byrne;A. Bakker;L. Simpson
锥虫线粒体RNA编辑的分子机制是一个尚未解决的问题。我们发现，两类核糖核蛋白复合物存在于利什曼原虫tarentolae的线粒体提取物，似乎参与RNA编辑。“G”类RNP复合物由170 - 300 A颗粒组成，其含有指导RNA和蛋白质，显示出很少的末端尿苷酰转移酶（TUTase）活性，并显示出体外RNA编辑样活性。“T”类由大约6个RNP复合物组成，其内源性RNA可以用[α-32 P]UTP自标记。最丰富的T复合物T-IV通过电子显微镜观察为80 - 140 A颗粒。该复合物在天然凝胶中表现出TUTase活性，并含有指导RNA。G和T复合物都可能参与体内RNA编辑。这些结果是分析RNA编辑生物化学的起点。
The molecular mechanism of RNA editing in trypanosomatid mitochondria is an unsolved problem. We show that two classes of ribonucleoprotein complexes exist in a mitochondrial extract from Leishmania tarentolae and appear to be involved in RNA editing. The ‘G’ class of RNP complexes consists of 170‐300 A particles which contain guide RNAs and proteins, show little terminal uridylyl transferase (TUTase) activity and exhibit an in vitro RNA editing‐like activity. The ‘T’ class consists of approximately six RNP complexes, the endogenous RNA of which can be self‐labeled with [alpha‐32P]UTP. The most abundant T complex, T‐IV, is visualized by electron microscopy as 80‐140 A particles. This complex exhibits TUTase activity in the native gel and contains guide RNAs. Both G and T complexes are possibly involved with RNA editing in vivo. These results are a starting point for the analysis of the biochemistry of RNA editing.