Periostin, a matricellular protein, accelerates cutaneous wound repair by activating dermal fibroblasts
Periostin, a matricellular protein, accelerates cutaneous wound repair by activating dermal fibroblasts
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DOI：
10.1111/j.1600-0625.2012.01454.x
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发表时间：
2012-05-01
影响因子：
3.6
通讯作者：
Naka, Tetsuji
中科院分区：
文献类型：
--
作者：
Ontsuka, Kanako;Kotobuki, Yorihisa;Naka, Tetsuji
皮肤创伤修复是一个高度有序和协调的过程，涉及各种细胞谱系和许多分子效应器。通过整合素分子的细胞基质相互作用为伤口修复提供了重要的关键信号。骨膜蛋白是一种基质细胞蛋白，其可通过磷脂酰肌醇3-激酶/Akt和MAP激酶途径与几种整合素分子相互作用，在组织发育和重塑期间提供重要信号。在这项研究中，我们研究了骨膜蛋白在皮肤伤口修复过程中的作用，使用骨膜蛋白缺陷的小鼠，并通过分析骨膜蛋白对真皮成纤维细胞的影响。我们首先确定了骨膜蛋白在一个良好表征的伤口修复模型小鼠中的表达谱和定位。骨膜蛋白大量沉积在肉芽组织下的扩展表皮伤口边缘和真皮表皮交界处受伤的小鼠。此外，骨膜蛋白缺陷小鼠表现出体内伤口修复延迟，这可以通过直接施用外源性骨膜蛋白来改善。体外分析表明，骨膜蛋白的损失损害真皮成纤维细胞的增殖和迁移，但外源性补充或加强骨膜蛋白的表达增强其增殖。总之，这些结果表明，骨膜蛋白通过激活成纤维细胞加速皮肤伤口修复的过程。
Cutaneous wound repair is a highly ordered and well-coordinated process involving various cell lineages and many molecular effectors. Cellmatrix interactions through integrin molecules provide key signals important for wound repair. Periostin is a matricellular protein that may provide signals important during tissue development and remodelling by interacting with several integrin molecules, via the phosphatidylinositol 3-kinase/Akt and MAP kinase pathways. In this study, we examined the role of periostin in the process of cutaneous wound repair using periostin-deficient mice and by analysing the effects of periostin on dermal fibroblasts. We first determined the expression profile and localization of periostin in a well-characterized wound repair model mice. Periostin was robustly deposited in the granulation tissues beneath the extended epidermal wound edges and at the dermalepidermal junctions in wounded mice. Moreover, periostin-deficient mice exhibited delayed in vivo wound repair, which could be improved by direct administration of exogenous periostin. In vitro analyses revealed that loss of periostin impaired proliferation and migration of dermal fibroblasts, but exogenous supplementation or enforced periostin expression enhanced their proliferation. Combined, these results demonstrate that periostin accelerates the process of cutaneous wound repair by activating fibroblasts.