Characterization of non-extractable 14C- and 13C-sulfadiazine residues in soil including simultaneous amendment of pig manure
Characterization of non-extractable 14C- and 13C-sulfadiazine residues in soil including simultaneous amendment of pig manure
复制标题
DOI：
10.1080/03601234.2011.535371
复制
发表时间：
2011-01-01
影响因子：
2
通讯作者：
Schmidt, Burkhard
中科院分区：
文献类型：
--
作者：
Junge, Thorsten;Meyer, Katrin Charlot;Schmidt, Burkhard
最近，我们报道了用 14C 标记的磺胺嘧啶 (14C-SDZ) 处理的猪粪改良 SDZ 残留物的土壤命运。本研究的首要目标是确定是否可以通过在土壤中施用 14C-SDZ 来替代该策略。第二个目标是通过分级、尺寸排阻色谱 (SEC) 和固态 13C-NMR 表征不可萃取的 SDZ 残留物。使用添加和未添加猪粪的两种土壤对 14C-SDZ 的命运进行了 28 天的检测。 14C-SDZ矿化度低；可提取残留物降低至 7-30%。与之前的研究相比，结果相似。在 HCl 洗涤液、黄腐酸、腐殖酸和胡敏素中发现了 14C-SDZ 衍生的结合放射性。根据 SEC，结合的 14C 部分 (70%) 与黄腐酸（高于 910 g mol-1）共洗脱；另一种由吸附/截留的 14C-SDZ 组成。 13C-SDZ研究进行30天；通过 13C-NMR 检查腐殖酸。信号(100-150 ppm)被称为13C-SDZ。 SEC 和 13C-NMR 证明 SDZ 可以快速整合到腐殖质中。
Recently, we reported on soil fate of SDZ residues amended with pig manure treated with 14C-labeled sulfadiazine (14C-SDZ). The first objective of the present study was to determine whether this strategy can be substituted by application of 14C-SDZ to soil. The second objective was to characterize non-extractable SDZ residues by fractionation, size exclusion chromatography (SEC) and solid state 13C-NMR. The fate of 14C-SDZ was examined for 28 d, using two soils with and without amendment of pig manure. Mineralization of 14C-SDZ was low; extractable residues decreased to 7-30%. Compared to the previous study, results were similar. 14C-SDZ derived bound radioactivity was found in HCl-washings, fulvic, humic acids and humin. According to SEC, one bound 14C portion (70%) co-eluted with fulvic acids (above 910 g mol-1); the other consisted of adsorbed/entrapped 14C-SDZ. The 13C-SDZ study was performed for 30 d; humic acids were examined by 13C-NMR. A signal (100-150 ppm) was referred to 13C-SDZ. SEC and 13C-NMR demonstrated rapid integration of SDZ into humics.