A biological approach to assembling tissue modules through endothelial capillary network formation.
A biological approach to assembling tissue modules through endothelial capillary network formation.
复制标题
通过内皮毛细血管网络形成组装组织模块的生物学方法。
DOI：
10.1002/term.2008
复制
发表时间：
2015
影响因子：
3.3
通讯作者：
Shen,Wei
中科院分区：
文献类型：
--
作者：
Riesberg,JeremiahJ;Shen,Wei
为了创建具有复杂结构的功能性组织，已经出现了将小组织模块组装成较大结构的自下而上的方法。这些方法中的大多数基于组织模块之间的界面处的化学反应或物理相互作用。在这里，我们报告了一种生物组装方法，通过内皮毛细血管网络的形成，整合小组织模块。当相邻的组织模块含有适当的细胞外基质材料和支持稳健的内皮毛细血管网络形成的细胞类型时，毛细血管小管形成并穿过界面生长，导致模块组装成单个较大的构建体。结果显示，在接种有人脐静脉内皮细胞（HUVEC）和间充质干细胞（MSC）的致密纤维蛋白凝胶模块中形成毛细血管网络;相邻模块牢固地组装成具有117 ± 26%的破坏应变、2208 ± 83 Pa的拉伸强度和2548 ± 574 Pa的杨氏模量的整合构建体。在相同的培养条件下，毛细血管网络是不存在的密集的纤维蛋白凝胶接种与HUVECs或MSC单独的模块;相邻的模块断开，即使轻轻地处理。这种生物组装方法消除了对化学反应或物理相互作用及其相关限制的需要。此外，整合的结构是预血管化的，因此这种自下而上的组装方法也可能有助于解决血管化问题，这是组织工程的另一个关键挑战。版权所有© 2015约翰威利父子有限公司.
To create functional tissues having complex structures, bottom‐up approaches to assembling small tissue modules into larger constructs have been emerging. Most of these approaches are based on chemical reactions or physical interactions at the interface between tissue modules. Here we report a biological assembly approach to integrate small tissue modules through endothelial capillary network formation. When adjacent tissue modules contain appropriate extracellular matrix materials and cell types that support robust endothelial capillary network formation, capillary tubules form and grow across the interface, resulting in assembly of the modules into a single, larger construct. It was shown that capillary networks formed in modules of dense fibrin gels seeded with human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs); adjacent modules were firmly assembled into an integrated construct having a strain to failure of 117 ± 26%, a tensile strength of 2208 ± 83 Pa and a Young's modulus of 2548 ± 574 Pa. Under the same culture conditions, capillary networks were absent in modules of dense fibrin gels seeded with either HUVECs or MSCs alone; adjacent modules disconnected even when handled gently. This biological assembly approach eliminates the need for chemical reactions or physical interactions and their associated limitations. In addition, the integrated constructs are prevascularized, and therefore this bottom‐up assembly approach may also help address the issue of vascularization, another key challenge in tissue engineering. Copyright © 2015 John Wiley & Sons, Ltd.