Effects of surface carboxylic acid groups of cerasomes, morphologically stable vesicles having a silica surface, on biomimetic deposition of hydroxyapatite in body fluid conditions
Effects of surface carboxylic acid groups of cerasomes, morphologically stable vesicles having a silica surface, on biomimetic deposition of hydroxyapatite in body fluid conditions
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DOI：
10.1007/s10856-009-3829-7
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发表时间：
2010-01-01
影响因子：
3.7
通讯作者：
Tanihara, Masao
中科院分区：
文献类型：
--
作者：
Hashizume, Mineo;Horii, Hiroyuki;Tanihara, Masao
仿生矿化是近年来生物材料研究的热点之一。研究了羟基磷灰石（HAp）在神经酰胺体（形态稳定的有机-无机杂化囊泡）上的仿生沉积。扫描电子显微镜，能量色散X-射线光谱和X-射线衍射研究表明，原始神经酰胺体诱导HAp的异质成核时，他们被浸泡在1.5SBF，具有1.5倍的离子浓度比模拟体液（SBF）的溶液。当二羧酸和一羧酸基团显示在cerasome表面上时，HAp沉积进一步加速。预期这些羧酸基团增强钙离子与神经酰胺酶体表面的结合，导致HAp成核位点的增加。在较低的表面浓度的cerasome表面上，二羧酸基团显然是更有效的HAp沉积比单羧酸基团。所得HAp-cerasome杂合体可用作具有源自神经酰胺体的脂质双层结构的独特性质的生物相容性材料。
Biomimetic mineralization of supramolecular scaffolds consisting of biomolecules or their analogues has received much attention recently from the viewpoint of creation of novel biomaterials. This study investigated biomimetic deposition of hydroxyapatite (HAp) on cerasomes, morphologically stable organic-inorganic hybrid vesicles. Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction studies revealed that the pristine cerasomes induced heterogeneous nucleation of HAp when they were immersed in 1.5SBF, a solution having 1.5 times higher ion concentration than that of a simulated body fluid (SBF). The HAp deposition was further accelerated when dicarboxylic and monocarboxylic acid groups were displayed on cerasome surfaces. These carboxylic acid groups are expected to enhance calcium ion binding to the cerasome surface, causing an increase of HAp nucleation sites. At lower surface concentrations on the cerasome surface, the dicarboxylic acid group is apparently more effective for HAp deposition than the monocarboxylic acid group. The resultant HAp-cerasome hybrids are useful as biocompatible materials having unique properties deriving from the lipid bilayer structure of the cerasomes.