Motility disorder in experimentally obstructed intestine: relationship between muscularis inflammation and disruption of the ICC network
Motility disorder in experimentally obstructed intestine: relationship between muscularis inflammation and disruption of the ICC network
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DOI：
10.1111/j.1365-2982.2005.00718.x
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发表时间：
2006-01-01
影响因子：
3.5
通讯作者：
Ozaki, H
中科院分区：
文献类型：
--
作者：
Won, KJ;Suzuki, T;Ozaki, H
我们设计了一种大鼠小肠梗阻模型，以研究与图尼卡肌层中免疫激活成分相关的肠收缩力变化。手术诱导肠梗阻后2-3周，模型大鼠扩张的肠道区域组织化学研究虽然没有发现任何典型的炎症体征，例如白细胞浸润，但图尼卡肌层中ED 2阳性巨噬细胞的数量似乎增加。肿瘤坏死因子（TNF）-α mRNA的表达也显著增加，图尼卡肌层中的CD 14水平也显著增加。扩张肠肌段的功能研究表明，胆碱能药物刺激的绝对力显着下降。此外，自发性节律性收缩的数量也减少了在膨胀的肠区域的阻塞肠，这种减少与卡哈尔间质细胞（ICC）的数量减少，所揭示的试剂盒样免疫反应。这些结果表明，在肠梗阻相关的图尼卡肌层的促炎条件下，可能来自活化的驻留巨噬细胞的生物活性物质的释放可能影响平滑肌收缩性。此外，在这种情况下，邻近的国际刑事法院的数量和功能也可能受到影响。
We designed a model of small intestinal obstruction in rats to investigate changes in intestinal contractility associated with the immunologically activated components in the tunica muscularis. Although histochemical study did not reveal any typical inflammatory signs such as leucocyte infiltration in the distended intestinal regions of model rats 2-3 weeks after surgical induction of intestinal obstruction, the number of ED2-positive macrophages appeared to be increased in the tunica muscularis. Expression of tumour necrosis factor (TNF)-alpha mRNA was also significantly increased, and the level of CD14 was also increased significantly in the tunica muscularis. Functional studies of distended intestinal muscle segments showed a marked decrease in absolute force stimulated by a cholinergic agent. In addition, the number of spontaneous rhythmic contractions was also reduced in the distended intestinal regions of the obstructed intestine, and this decrease was associated with a reduction in the number of interstitial cells of Cajal (ICC), as revealed by Kit-like immunoreactivity. These results suggest that, under the pro-inflammatory conditions of the tunica muscularis associated with intestinal obstruction, the release of bioactive substances, possibly from activated resident macrophages, may affect smooth muscle contractility. Furthermore, under these conditions, both the number and the function of neighbouring ICC may also be affected.