Clinical relevance of CD44 cell-surface expression and N-myc gene amplification in a multicentric analysis of 121 pediatric neuroblastomas
Clinical relevance of CD44 cell-surface expression and N-myc gene amplification in a multicentric analysis of 121 pediatric neuroblastomas
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DOI：
10.1200/jco.1996.14.1.25
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发表时间：
1996-01-01
影响因子：
45.3
通讯作者：
Favrot, MC
中科院分区：
文献类型：
--
作者：
Combaret, V;Gross, N;Favrot, MC
目的：与其他人类肿瘤相反，神经母细胞瘤细胞表面糖蛋白CD 44的抑制是侵袭性的标志，通常与N-myc扩增相关。因此，我们比较了两种标志物在121名儿童治疗的神经母细胞瘤在协作institutions.Methods的初始分期的预后价值：冷冻样本进行了分析，通过一个快速和良好的标准化的免疫染色技术与单克隆抗体（单克隆抗体）对抗原决定簇的CD 44恒定区。在这一回顾性研究中，CD 44在102例标本中表达，并与良好的肿瘤分期和组织学、年轻和正常的N-myc拷贝数密切相关。在单变量分析中，CD 44表达和正常N-myc是有利临床结果的最有力标志物（分别为P < 10（-6）和chi（2）= 65.40; P < 10（-6）和chi（2）= 42.56），但CD 44分析在有或无N-myc扩增肿瘤的患者亚组中提供了显著的预后区分。在IV期神经母细胞瘤的亚组中，CD 44是唯一有意义的预后标志物（P <0.02，chi（2）= 5.76），而N-myc状态不是判别性的。在N-myc扩增、CD 44表达、年龄、肿瘤分期和组织学5个因素的多因素分析中，CD 44表达和肿瘤分期是无事件生存期的唯一独立预后因素。结论：CD 44细胞表面表达的分析可作为肿瘤早期分期的一项生物学指标。
Purpose: In contrast to other human tumors, a repression of the cell-surface glycoprotein CD44 on neuroblastoma is a marker of aggressiveness that usually correlates to N-myc amplification. We thus compared the prognostic value of both markers in the initial staging of 121 children treated for neuroblastoma in collaborative institutions.Methods: Frozen samples were analyzed by a rapid and well-standardised technique of immunostaining with monoclonal antibodies (MoAbs) against epitopes in the CD44 constant region.Results: In this retrospective series, CD44 was expressed 102 specimens and strongly correlated with favorable tumor stages and histology, younger age, and normal N-myc copy numbers. In univariate analysis, CD44 expression and normal N-myc were the most powerful markers of favorable clinical outcome (P < 10(-6) and chi(2) = 65.40; and P < 10(-6) and chi(2) = 42.56, respectively), but analysis of CD44 affords significant prognostic discrimination in subgroups of patients with or without N-myc-amplified tumors. In the subgroup of stage IV neuroblastomas, CD44 was the only significant prognostic marker (P < .02, chi(2) = 5.76), whereas N-myc status was not discriminant. In multivariate analysis of five factors, ie, N-myc amplification, CD44 expression, age, tumor stage, and histology, the only independent prognostic factors of event-free survival were CD44 expression and tumor stage.Conclusion: The analysis of CD44 cell-surface expression must be recommended as an additional biologic marker in the initial staging of the disease.