Zinc (Zn) Doping by Hydrothermal and Alkaline Heat-Treatment Methods on Titania Nanotube Arrays for Enhanced Antibacterial Activity.
Zinc (Zn) Doping by Hydrothermal and Alkaline Heat-Treatment Methods on Titania Nanotube Arrays for Enhanced Antibacterial Activity.
复制标题
水热和碱性热处理方法的锌（Zn）掺杂，以增强抗菌活性。
DOI：
10.3390/nano13101606
复制
发表时间：
2023-05-10
期刊：
影响因子：
--
通讯作者：
Popat KC
中科院分区：
文献类型：
--
作者：
Bhattacharjee A;Goodall E;Pereira BL;Soares P;Popat KC
钛(Ti)具有优异的耐腐蚀性和低弹性模数等力学性能，是一种广泛应用于骨科植入物的生物材料。然而，每年约有10%的种植体因细菌感染和骨结合不良而失败，导致患者严重疼痛和痛苦。为了改善其性能，可以利用纳米级的表面修饰方法和表面掺杂微量元素，这可能有助于改善细胞粘附性，从而在减少细菌感染的同时更好地实现骨整合。在本工作中，首先通过阳极氧化在商用纯钛表面制备了二氧化钛纳米管阵列(NT)。然后采用两种不同的方法进行锌掺杂：水热处理和碱性热处理。扫描电子显微镜(SEM)图像显示了独特的表面形貌，而能量色散X射线能谱(EDS)显示了锌在表面的分布。接触角测量表明，NT表面具有超亲水性。X射线光电子能谱(XPS)提供了表面锌的相对含量，表明水热处理的表面比碱热处理的表面含有更多的锌。X射线结晶学和纳米压痕技术提供了表面的晶体结构和机械性能。当用脂肪干细胞(ADSC)进行测试时，表面对细胞没有明显的细胞毒性。培养6 h和24 h后，观察细菌在表面的黏附和形态变化。结果表明，与钛对照组相比，掺锌纳米管表面的细菌黏附性显著降低。因此，掺锌纳米管表面为骨科植入物的应用提供了潜在的平台。
Titanium (Ti) is a popular biomaterial for orthopedic implant applications due to its superior mechanical properties such as corrosion resistance and low modulus of elasticity. However, around 10% of these implants fail annually due to bacterial infection and poor osseointegration, resulting in severe pain and suffering for the patients. To improve their performance, nanoscale surface modification approaches and doping of trace elements on the surfaces can be utilized which may help in improving cell adhesion for better osseointegration while reducing bacterial infection. In this work, at first, titania (TiO2) nanotube arrays (NT) were fabricated on commercially available pure Ti surfaces via anodization. Then zinc (Zn) doping was conducted following two distinct methods: hydrothermal and alkaline heat treatment. Scanning electron microscopic (SEM) images of the prepared surfaces revealed unique surface morphologies, while energy dispersive X-ray spectroscopy (EDS) revealed Zn distribution on the surfaces. Contact angle measurements indicated that NT surfaces were superhydrophilic. X-ray photoelectron spectroscopy (XPS) provided the relative amount of Zn on the surfaces and indicated that hydrothermally treated surfaces had more Zn compared to the alkaline heat-treated surfaces. X-ray crystallography (XRD) and nanoindentation techniques provided the crystal structure and mechanical properties of the surfaces. While testing with adipose-derived stem cells (ADSC), the surfaces showed no apparent cytotoxicity to the cells. Finally, bacteria adhesion and morphology were evaluated on the surfaces after 6 h and 24 h of incubation. From the results, it was confirmed that NT surfaces doped with Zn drastically reduced bacteria adhesion compared to the Ti control. Zn-doped NT surfaces thus offer a potential platform for orthopedic implant application.